摘要
目的将人单核细胞系U937及THP-1体外诱导得到M2型巨噬细胞。方法将U937及THP-1细胞以phorbol 12-Myristate 13-Acetate(PMA)及白介素-4(interleukin,IL-4)序贯诱导,采用q RT-PCR法及ELISA法检测M1/M2标志物m RNA及蛋白表达。结果 PMA使两种细胞转化为巨噬细胞,IL-4序贯诱导使巨噬细胞伸出伪足,序贯诱导后巨噬细胞M1表型标志物(IL-1β、IL-6、IL-12及i NOS)m RNA表达水平下调,M2表型标志物(IL-10、CD163)m RNA表达水平上调。同时,CCL18蛋白和m RNA表达水平均上调,而雷帕霉素可下调升高的CCL18表达水平。结论及IL-4序贯诱导人单核细胞系成为M2型巨噬细胞,雷帕霉素可逆转这一过程。
Objective To study if U937 and THP-1 human monocyte cell lines were induced into the M2 type macrophages by in vitro stimulation, and to explore the effect of rapamycin on this process. Methods The U937 and THP-1 cell lines were stimulated by phorbol 12-Myristate 13-Acetate（PMA） and interleukin-4（IL-4） sequentially. The expressions of M1/M2 markers protein and m RNA in the stimulated macrophages were examined by q RT-PCR and ELISA, respectively. Results Both of U937 and THP-1 human monocytes were induced into macrophages by PMA, and with pseudopods further by the subsequent stimulation with IL-4. The expression levels of M1 associated markers（IL-1β、IL-6、IL-12 and i NOS） m RNA were downregulated significantly（ 0.01）, but the expression levels of M2 associated markers（IL-10、CD163） m RNA were upregulated significantly. At the same time, the expression level of CCL18 protein and m RNA was highly elevated by the stimulation of IL4, but the elevated CCL18 expression level was downregulated by further stimulation of rapamycin. Conclusion The sequential stimulation by PMA and IL4 induces human monocytes into M2 type macrophages, but rapamycin could reverse this process.
出处
《解剖科学进展》
CAS
2015年第3期294-298,共5页
Progress of Anatomical Sciences
基金
国家自然科学基金(No.81301767)
辽宁省高等学校重大科技平台免疫皮肤病重点实验室自主创新课题基金
辽宁省教育厅项目(No.L2014294)
沈阳市科技计划项目(No.F15-199-1-42)
