摘要
为弄清贵州地方山羊脂肪细胞型脂肪酸结合蛋白(adipocyte fatty acid-binding protein,A-FABP)基因mRNA的表达规律,奠定肉质性状分子标记辅助选择的理论基础,试验以β-actin基因为内参,应用实时荧光定量PCR技术检测黔东南小香羊、贵州白山羊、贵州黑山羊、黔北麻羊和南江黄羊共5个山羊品种心脏、肝脏、肺脏、肾脏、背最长肌、半膜肌及皮下脂肪7个组织器官中A-FABP基因的表达。结果表明,5个山羊品种7个组织中都有A-FABP基因表达,且5个品种中皮下脂肪A-FABP基因的表达水平都较高;在贵州地方山羊A-FABP基因表达的最主要组织——皮下脂肪,以及在与产肉性能直接相关的组织——背最长肌和半膜肌中,黔东南小香羊A-FABP基因mRNA水平都较高。结果提示,贵州地方山羊A-FABP基因mRNA的组织分布模式相似,黔东南小香羊的优良肉质特性或许与其皮下脂肪、背最长肌和半膜肌中A-FABP基因的高表达有关。
Abstract： In order to understand the regularities of A-FABP gene expression in Guizhou native goats, and to establish the theoretical basis of molecular marker assisted selection for meat quality traits,β-actin gene was used as control, expression levels of A-FABP gene in livers, kidneys, hearts, lungs, longissimus muscle, semimembranosus muscle and subcutaneous fat of Qiandongnan small Xiang goat,Guizhou White goat,Guizhou Black goat,Qianbei Ma goat and Nanjiang Yellow goat were detected by Real-time fluorescence quantitative PCR. The results showed that A-FABP gene was expressed in all 7 tissues of 5 goat breeds, and the expression levels of A-FABP gene mRNA were all high in subcutaneous fat of 5 goat breeds^In subcutaneous fat, which was the main tissue of A-FABP gene expression in Guizhou native goats, and in longissimus muscle and semimembranosus muscle, which were directly associated with meat production property, A-FABP gene mRNA levels were all high in Qiandongnan small Xiang goat. The results sugges- ted that the tissue distribution patterns of A-FABP gene mRNA in Guizhou native goat breeds were similar to each other. The excellent meat quality of Qiandongnan small Xiang goat might be related with the high expression levels of A-FABP gene mRNA in it＇s subcutaneous fat,longissi- mus muscle and semimembranosus muscle.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第6期1538-1546,共9页
China Animal Husbandry & Veterinary Medicine
基金
凯里学院规划课题(Z1304)
凯里学院获得博士学位(引进教授)教师专项课题(BS201303)
贵州省科技联合基金项目(黔科合J字LKK[2013]14号)
贵州省普通本科高校自然科学研究项目(黔教合KY字[2014]236)
国家自然科学基金(81460572)
