摘要
目的研究不同渗透压液体洗胃对胃黏膜、血浆渗透压（POP）、血浆前列腺素E2（PGE2）和内皮素-1（ET-1）的影响，探讨血浆PGE2、ET-1和POP等参数对洗胃导致胃黏膜损伤程度评估的价值与意义。方法将32只日本长耳大白兔随机分为正常对照组（NC组）、清水洗胃组（CW组）、0．45％氯化钠溶液洗胃组（HS组）、0．9％氯化钠溶液洗胃组（NS组），每组8只。CW组、HS组、NS组分别用清水、0．45％氯化钠溶液和0．9％氯化钠溶液洗胃；NC组仅置胃管不洗胃。在置胃管前及洗胃后，采动脉血液标本测定血浆PGE2、ET-1和POP；然后用气栓法处死兔，取出胃，在肉眼和光镜下评价胃黏膜损伤程度。结果洗胃前各组POP、PGE2以及ET-1相比，差异无统计学意义。洗胃后，CW组和HS组POP显著下降，显著低于NC组和NS组，差异有统计学意义（P〈0．05）；NS组、HS组和CW组血浆PGE2浓度显著升高，显著高于NC组，差异有统计学意义（P〈0．05）；NS组、HS组和CW组血浆ET-1浓度显著升高，高于NC组，差异有统计学意义（P〈0．05）。NS组、HS组和CW组DI／EDS显著高于NC组，差异有统计学意义（P〈0．05）。相关性分析表明，洗胃后POP与胃黏膜损伤程度呈显著负相关，血浆PGE2和ET-1与胃黏膜损伤程度呈显著正相关。结论不同渗透压液体洗胃可导致家兔POP、血浆PGE2和ET-1浓度的改变，这种改变可在一定程度上反映胃黏膜损伤程度。
Objective To explore the effect of gastrolavage with different osmotic pressure solutions on gastric mucosa, plasma osmotic pressure, plasma PGE2 and ET-1 in rabbits, and investigate the value and meaning of the parameters such as plasma PGE2, ET-1 and POP for the evaluation of gastric mucosa damage degree. Methods A total of 32 rabbits were randomly divided into four groups with 8 in each group： control （NC） group, clear water （CW） group, 0.45% sodium chloride solution （HS） group, and 0.9% sodium chloride solution （NS） group. After the rabbits were anesthetized, they were given gastric lavage with homemade improvised gastric lavage apparatus. The blood samples of each group were collected at pre-gastrolavage and post-gastrolavage. PGE2 and ET-I in plasma were measured by ELISA. At the end of the experiment, the rabbits＇ stomach were removed after being sacrificed. The injury level of the gastric mucosa was observed by macroscopic and light. Results Before gastrolavage, plasma osmotic pressure, PGE2 and ET-1 did not show any significant difference among the groups （P 〉0.05）. After gastrolavage, plasma osmotic pressure in CW group and HS group significantly decreased and were lower than that in NC group and NS group （P 〈0.05）. The expression value of plasma PGE2 in NS group, HS group and CW group significantly increased and were higher than that in NC group （P 〈0.05）. The expression value of plasma ET-1 in NS group, HS group and CW group significantly increased and were higher than that in NC group （P 〈0.05）. The gastric mucosa DI and EDS in NS group, HS group, CW group were significantly higher than that in NC group （P 〈0.05）. Correlation analysis reflected that after gastrolavage POP and gastric mucosa damage degree were significantly negative correlated, while plasma PGE2/ET-1 and gastric mucosa damage degree were significantly positive correlated. Conclusion Gastrolavage with different osmotic pressure solution may result in the changes of POP, plasma PGE2 and ET-1 in rabbit, and the changes may reflect the degree of gastric mucosa damage.
出处
《中国急救复苏与灾害医学杂志》
2015年第6期526-530,共5页
China Journal of Emergency Resuscitation and Disaster Medicine
关键词
洗胃液
渗透压
黏膜损伤
前列腺素E2
内皮素-1
Rabbit
Gastric lavage
Plasma osmotic pressure
Gastric mucosa damage
Prostaglandin E2
Endothelin- 1
