摘要
建立了呋喃它酮代谢物5-吗啉甲基-3-氨基-2-唑烷基酮（AMOZ）间接竞争化学发光酶免疫分析（ic CLEIA）检测方法,通过单因素实验优化了包被原浓度、抗体稀释倍数、反应缓冲体系及浓度、竞争反应时间等参数,结果表明ic CLEIA最佳反应条件为：包被抗原浓度为10 ng/m L,抗体稀释60000倍,最佳竞争时间为50 min,体系缓冲液0.01 mol/L PBS（p H 7.4）。在优化的条件下,本方法的线性检测范围为0.026~3.52μg/L,IC50为0.29μg/L,检出限（LOD,IC10）为0.012μg/L。对鱼肉样品的平均添加回收率在101.4%~115.5%之间。建立的ic CLEIA方法可用于实际样品中AMOZ残留检测。
Indirect competitive chemiluminescence enzyme immunoassay （icCLEIA） detection for furaltadone metabolite 5-morpholinomethyl-3-amino-2-oxazolidone （AMOZ） was established. The effects of the coating antigen concentration, antibody dilution, immunoreaction time, reaction buffer system on the performance of the assay were studied and optimized. The result showed that the optimized assay conditions were as follows： the coating antigen concentration was 10 ng/mL; the polyclonal antibody was diluted 60000 times, immunoreaction time was 50 min and the buffer solution was 0.01 mol/L phosphate buffer solution （ pH 7.4）. Under the optimal conditions, the linear detection range of the developed ieCLEIA was 0. 026-3.52 μg/L, the IC50 was 0.29 μg/L and the limit of detection （ IC10 ） was 0. 012 μg/L. The average recoveries of AMOZ of spiked fish ranged from 101.4% to 115.5%. The icCLEIA is applicable for the detection of trace AMOZ in fish samples.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2015年第6期871-875,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(Nos.31371769,No.31271865)
广东省科技计划项目(Nos.2012A020100002,No.2013B040402006)
广州市科技计划项目(No.2013J4100053)资助~~
