摘要
为探讨普安银鲫早期发育过程中转铁蛋白基因表达特点和维生素C溶液浸泡对其表达量的影响,分别采用RT-PCR与荧光定量PCR技术研究了维生素C对普安银鲫早期发育中转铁蛋白基因表达的影响。试验结果表明,在成熟卵,受精卵和囊胚晚期中转铁蛋白基因均未表达,发育至原肠中期开始表达,之后呈"升高—降低—升高"的趋势,在心跳期达到最高峰,出膜前期最低;在30mg/L的维生素C处理组中,胚胎期转铁蛋白基因的表达量低于正常组,而出膜以后则高于正常组。研究表明,转铁蛋白在胚胎期的功能是独立于其结合铁之外的功能,而维生素C的添加降低了转铁蛋白在胚胎期参与免疫等方面的功能,增强了其在仔鱼中运输和转运铁离子的功能。
In order to discuss the gene expression characteristics of transferrinand effects of vitamin C so lution on the expression of transfcrrin genes during early development of crucian carp Carassius auratus gibelio. RT-PCR and fluorescence quantitative PCR techniques were used to detect effect of vitamin C on the expression of transferrin genes and its mRNA expression Ievels during the early development of crucian carp. The results showed no expression of transferrin in mature eggs, fertilized eggs and expanded blasto cyst stage, while the expression of transferrin was determined in gastrula stage, and the mRNA expression level of transferrin showed ＂up-down up＂ trend, and a peak in the heatbeating stage, and the minimum in the prehatching stage. In the group of vitamin C（30 mg/L）, the mRNA expression level of transferrin was lower than that in normal group during embryonic stage, while higher than that in normal group during larval stage. The findings showed that the function of transferrin was independent from the binding iron function during embryonic development, and appropriate vitamin C led to be decreased in the function of transferrin in immune during embryonic development, and to be increased in the function of transport iron in yolk-sac larval stage.
出处
《水产科学》
CAS
CSCD
北大核心
2015年第6期370-374,共5页
Fisheries Science
基金
国家自然科学基金资助项目(31160527)
贵州大学研究生创新基金资助项目(研农2014014)
关键词
普安银鲫
早期发育
转铁蛋白
基因表达
荧光定量
维生素C
Carassius auratus gibelio
early development
transfcrrin
gene expression
qPCR
vitamin C
