摘要
目的：探讨白细胞介素2（IL-2）以及肿瘤坏死因子-α（TNF-α）基因启动子SNP位点与丙型肝炎病毒（HCV）慢性感染的相关性。方法：选择云南地区汉族人群HCV慢性感染患者380例,健康体检人群367例,采用Taq Man探针基因分型方法对IL-2基因启动子SNPrs2069762、rs2069763和rs4833248,以及TNF-α基因启动子SNPrs1800629、rs3093668和rs3093726进行基因分型,并构建单倍型,统计IL-2和TNF-α多态性位点的等位基因频率与基因型频率、单倍型频率,分析SNPs及单倍型与HCV慢性感染的相关性。结果：IL-2基因启动子中,病例组和对照组相比,SNPrs2069762基因型差异有统计学意义（P〈0.05,OR=0.864,95%CI为0.700~1.066）,病例组CC基因型频率高于对照组（P〈0.05）,rs2069763等位基因差异具有统计学意义（P〈0.05,OR=1.234,95%CI为1.007~1.513）,病例组等位基因G频率高于对照组（P〈0.05）,rs4833248基因型和等位基因频率差异无统计学意义（P〉0.05）,rs2069762/rs2069763/rs4833248单倍型ATG差异有统计学意义（P〈0.05,OR=0.812,95%CI为0.662~0.996）,病例组单倍型ATG频率低于对照组（P〈0.05）;TNF-α基因启动子SNPrs1800629、rs3093668和rs3093726基因型、等位基因型和单倍型频率,在病例组和对照组中差异无统计学意义（P〉0.05）。结论：在云南汉族群体中,IL-2基因启动子SNPrs2069762 CC基因型和rs2069763 G等位基因可能是HCV慢性感染的易感因素,rs2069762/rs2069763/rs4833248单倍型ATG可能是HCV慢性感染的保护性因素;TNF-α基因启动子SNPrs1800629、rs3093668和rs3093726与HCV慢性感染没有相关性。
Objective： To investigate the correlation among HCV chronic infection and SNPs in IL-2and TNF-α gene promoter. Method： A total of 380 patients with HCV chronic infection and 367 Healthy controls of Yunnan Han people were selected. Taq Man probe genotyping was applied to detect the IL-2 gene promoter SNP rs2069762,rs2069763 and rs4833248,coupled with TNF-α gene promoter SNP rs1800629,rs3093668,rs3093726,then constructing haplotype. Estimating allele frequency and genotype frequency,and haplotype frequency of IL-2 and TNF-αSNP. Then analyzing correlation between SNPs and haplotype AGT and HCV chronic infection. Results： Concerning IL-2 gene promoter of infection group and control group,SNP rs2069762 genotypic variation showed significant difference（ P 0. 05,OR = 0. 864,95% CI： 0. 700 ~ 1. 066）,and allele frequency of genotype CC in HCV chronic infection patients was higher than that of control group（ P 0. 05）; the rs2069763 allele showed a significant difference（ P 0. 05,OR = 1. 234,95% CI1. 007 ~ 1. 513） and the allele G frequency of infection group was higher than that of control group（ P 0. 05）; the genotype and allele frequency of rs4833248 displayed no significant difference（ P 0. 05）; rs2069762 / rs2069763 /rs4833248 haplotype ATG showed significant difference（ P 0. 05,OR = 0. 812,95% CI： 0. 662 ~0. 996） and haplotype ATG frequency of infection group was lower than control group（ P 0. 05）.Genotype,allele and haplotype frequency of rs1800629,rs3093668,rs3093726 in TNF-α had no statistical significance in both groups（ P 0. 05）. Conclusions： For Han people in Yunnan province,the rs2069762 genotype CC and rs2069763 allele G in IL-2 are potential predisposing factors of HCV chronic infection. rs2069762 / rs2069763 / rs4833248 haplotype ATG might play a protective role in HCV chronic infection. SNP rs1800629,rs3093668,rs3093726 in TNF-α gene promoter show no correlation with HCV chronic infection.
出处
《贵阳医学院学报》
CAS
2015年第6期571-575,587,共6页
Journal of Guiyang Medical College
基金
国家自然科学基金资助项目(81160197)
吴阶平医学基金项目(300.6750.13395)
