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支气管哮喘患者 Th17细胞的检测及其意义 被引量：2

Detection of Th17 cell in patients with bronchial asthma and itssignificance
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摘要 目的：检测支气管哮喘患者外周血、肺泡灌洗液中 Th17的表达水平，并探讨其在支气管哮喘发病中的作用及意义。方法收集支气管哮喘急性发作患者52例作为观察组，并且将上述患者根据严重度再分组（重度、中度、轻度）；另外选取同时期在该院进行健康体检者50例作为对照组，收集上述病例的外周血及肺泡灌洗液，采用实时荧光定量 PCR 法检测 Th17细胞的特异性转录因子 RORγt 及细胞因子 IL-17在外周血及肺泡灌洗液中的表达水平。结果观察组外周血及肺泡灌洗液中的 IL-17、RORγt 的相对表达量均高于对照组（外周血 IL-17表达量：观察组重度、中度、轻度分别为（1．89±0．09）、（1．02±0．1）、（1．11±0．09），对照组为（0．69±0．12），外周血 IL-17mRNA，重度组与对照组比较，t ＝2．891，P ＝0．001；中度组与对照组比较，t ＝1．923，P ＝0．023；轻度组与对照组比较，t ＝2．011，P ＝0．019；重度组与中度组比较，t ＝2.014，P ＝0.038；重度组与轻度组比较，t ＝1．911，P ＝0．031；中重度组与轻度组比较，t ＝1．021，P ＝0．089；外周血 RORγt表达量：观察组重度、中度、轻度分别为（1．42±1．71）、（0．89±2．32）、（0．91±1．99），对照组为（0．53±2.06），外周血 RORγtmRNA，重度组与对照组比较，t ＝2．723，P ＝0．021；中度组与对照组比较，t ＝1．993，P ＝0．015；轻度组与对照组比较，t ＝1．782，P ＝0．037；重度组与中度组比较，t ＝1．875，P ＝0．041；重度组与轻度组比较，t ＝2．016，P ＝0．038；中重度组与轻度组比较，t ＝1．023，P ＝0．714；肺泡灌洗液 IL-17表达量：观察组重度、中度、轻度分别为（2．35±1．09）、（1．92±0．21）、（2．01±0．12），对照组为（0．76±0．31），BALF 中IL-17mRNA，重度组与对照组比较，t ＝2．872，P ＝0．001；中度组与对照组比较，t ＝2．51，P ＝0．021；轻度组与对照组比较，t ＝1．790，P ＝0．037；重度组与中度组比较，t ＝1．689，P ＝0．031；重度组与轻度组比较，t ＝1．862， P ＝0．036；中重度组与轻度组比较，t ＝1．031，P ＝0．102；肺泡灌洗液 RORγt 表达量：观察组重度、中度、轻度分别为（2．13±1．42）、（1．42±1．72）、（1．51±2．01），对照组为（0．62±0．25），BALF 中 RORγtmRNA，重度组与对照组比较，t ＝2．721，P ＝0.021；中度组与对照组比较，t ＝2．811，P ＝0．029；轻度组与对照组比较，t ＝1.973，P ＝0．033；重度组与中度组比较，t ＝1．759，P ＝0．041；重度组与轻度组比较，t ＝1．918，P ＝0．039；中重度组与轻度组比较，t ＝1．014，P ＝0．055）；且观察组中重度患者的外周血及肺泡灌洗液中的 IL-17、RORγt 的相对表达量高于中、轻度患者，且差异均有统计学意义（P ＜0．05）。结论支气管哮喘患者的体内存在辅助T 淋巴细胞17（Th17）优势表达，Th17可能在支气管哮喘的发病机制中发挥重要作用。 Objective To detect the expression of Th17 levels about peripheral blood in patients with bron-chial asthma in bronchoalveolar lavage fluid,and to investigate its effect in pathogenesis of bronchial asthmaand. Methods Patients with acute bronchial asthma from 2014 April to 2014 November were collected in the experiment. There are 52 cases as observation group （group of patients with asthma）,and the patients were then divided according the severity （mild,moderate,severe）;in addition,select 50 healthy people in the same period in our hospital as con-trol group healthy control group.Collect peripheral blood and bronchoalveolar lavage fluid,the expression of specific transcription factors ROR gamma T and cytokine IL -17 in the peripheral blood and bronchoalveolar lavage fluid in Th17 cells levels were detected by real -time fluorescent quantitative PCR.Results The expression of IL -17,ROR gamma T in peripheral blood and bronchoalveolar lavage fluid of the observation group were higher than the control group;the expression of IL -17,ROR gamma T in alveolar lavage fluid quantity and peripheral blood with severe patients in the observation group were higher than that in the mild patients,differences were statistically significant （P 〈0.05）.Conclusion There are 17 auxiliary T lymphocytes in patients with bronchial asthma in vivo （Th17） advantage of expression,Th17 may play an important role in the pathogenesis of bronchial asthma.Bronchial asthma patients body may have advantage to heip expression of T lymphocytes 17 （Th17）.Th17 may play an important role in the pathogenesis of bronchial asthma.
作者 史嵘 乔珊 王韵 月珍
机构地区 北京大学深圳医院特诊病房
出处 《中国基层医药》 CAS 2015年第13期2010-2013,共4页 Chinese Journal of Primary Medicine and Pharmacy
关键词 支气管哮喘 辅助 T 淋巴细胞 IL-17 发病机制 聚合酶链反应 Bronchial asthma T helper cell IL -17 Pathogenesis Polymerase chain reaction
分类号 R562.25 [医药卫生—呼吸系统]
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参考文献14

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共引文献2073

同被引文献24

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中国基层医药
2015年 第13期

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