摘要
目的:验证胶质瘤细胞(C6)可能的一种乳酸快速排泌机制。方法:C6按6组不同浓度(0.5×105;0.75×105;1×105;1.25×105;1.5×105;2×105)接种,24h收集上清液,检测乳酸,乳酸脱氢酶含量,以海马神经元HT22作为对照。结果:接种浓度为1×105个/L组细胞24h密度适中处于指数增生期,乳酸和乳酸脱氢酶含量高于其他组(P<0.01)(除1.25×105个/L浓度组)。C6细胞培养上清液中乳酸含量明显高于HT22细胞培养上清液(P<0.01)。结论:胶质瘤细胞可能有一种快速排泌机制,使得其代谢产生的大量乳酸在细胞外液中堆积。
Objective： To validate the quick exhaust urinary mechanism of lactic acid glioma cell.Methods：C6cells were inoculated according to different concentrations in 6 groups（0.5 × 10^5; 0.75 × 10^5; 1 × 10^5; 1.25 ×10^5; 1.5 × 10^5; 2 × 10^5）.The supernatant was collected after 24 hours.The contents of lactic acid and lactate dehydrogenase were determinated with hippocampal neurons HT22 as control.Results： The number of cells is moderate and cells grow at an exponential rate in the group of 1 × 10^5/ L inoculation concentration after 24 h.Lactic acid and lactate dehydrogenase were higher than those in other groups（P 0.01）（except for 1.25 × 10^5 concentration group）.Lactic acid content of C6 cell culture supernatant was significantly higher than that in HT22cell（P 0.01）.Conclusion： Glioma cells may have a rapid excretion mechanism.The accumulation of large amounts of lactic acid makes the metabolized in the extracellular fluid.
出处
《黑龙江医药科学》
2015年第3期44-45,47,共3页
Heilongjiang Medicine and Pharmacy
基金
佳木斯大学研究生科技创新面上项目
编号:LM2014-023
黑龙江省卫生厅项目
编号:2011-401
关键词
脑胶质瘤
细胞培养
乳酸
乳酸脱氢酶
glioma
cell culture
lactic acid
lactic acid dehydrogenase
