摘要
目的 观察槲皮素对矽肺纤维化大鼠巨噬细胞（AM）和核因子-κB（NF-κB）信号通路的影响。方法 90只SPF级Wistar雄性大鼠,随机分为对照组（NS组）、模型组（M组）、槲皮素组（Que组）,每组30只。M组与Que组大鼠采用非暴露法气管内一次性注入SiO2悬液（250mg/kg）复制动物矽肺纤维化模型;NS组以同样方法注入生理盐水0.2~0.3ml。3组分别在注射SiO2后第1、3、7、14、21、28天处死动物。收集支气管肺泡灌洗液（BALF）和血清后,应用血细胞计数板计数AM总数,酶联免疫吸附测定（ELISA）法检测大鼠血清中肿瘤坏死因子-α（TNF-α）的含量,免疫组织化学法检测肺组织中NF-κB表达。结果 M组自第1天开始BALF中AM总数均不同程度增高,于第3天达高峰,第28天降至正常NS组水平。Que组AM总数各时间点与M组对应时间点比较均降低,第1、3、7、14、21天差异有统计学意义（P〈0.05）。与NS组比较,M组大鼠血清中的TNF-α含量各时间点明显升高,差异有统计学意义（P〈0.05）;Que组TNF-α含量各时间点均升高,与同时期M组、NS组比较,差异有统计学意义（P〈0.05）。M组大鼠肺组织中NF-κB蛋白表达高峰在第7天,各时间点均高于NS组及Que组,两两比较差异均有统计学意义（P〈0.05）。Que组大鼠肺组织中NF-KB的表达较模型组减少,但高于NS组,各时间点两两比较,差异有统计学意义（P〈0.05）。结论槲皮素通过抑制NF-κB通路减少大鼠炎症细胞、炎症介质TNF-α等释放,减轻炎症反应过程,缓解肺纤维化过程。
Objective To explore the effect of Quercetin on macrophages and nuclear factor kappa B in the lung of silicotic rat. Methods Totally 90 male Wistar rats, class SPF, were randomly divided into three groups： saline chloride control group （group NS）, model group （group M） and Quercetin group （group Que）, （each group had 30 rats）. Pulmonary fibrosis of rat model was induced by intratracheal instillation of SiO2, while the rats in group NS were given sodium chloride in the same condition. Bronchoalveolar lavage fluid （BALF） and serum were collected on the first, 3th, 7th, 14th, 21th, 28th day after the injection of SiO2 and the rats in all groups were put to death. The total number of AM was counted by blood cell counting plate. The content of TNF-a in blood serum was determined by ELISA. The The expression of nuclear factor kappa B in lung tissue were investigated by immunohistochemical. Results The total number of AM in group M increased from the first, up to peak on the third days, then went back to the level of group NS on the 28th days. At the same time the total AM in group Que at each time point was less than in group M, the difference was significant on the first days, 3 days, 7 days, 14 days, 21 days（P〈0. 05）. Compared with the saline chloride control group, the content of TNF-α in blood serum of the rats in group M on all the time points was higher （P 〈 0. 05） ; the content of TNF-a in blood serum of the rats in group Que on all the time points was higher than that of group NS and group M （P〈0. 05）. （3） Expression of NF-κB protein in lung tissue of rats in group M was higher than that of group NS and group Que （P〈0.05） ; the expression of NF-κB protein in group M peak in seventh days. Expression of NF-κB in lung tissue of rats with group Que was decreased compared to the group M, but higher than that of group NS at each time point （P〈 0. 05）. Conclusions Quereetin could reduce rat inflammatory cells and mediators （TNF-α） and then alleviate pulmonary fibrosis through inhibition of NF-κB pathway.
出处
《工业卫生与职业病》
CAS
2015年第4期275-279,共5页
Industrial Health and Occupational Diseases
基金
唐山市科技局科研基金资助(14130262B)
