摘要
通过单一因素试验并结合SPSS软件分析,得出对嗜酸乳杆菌的生长有较大影响的条件,通过响应面优化,得到嗜酸乳杆菌秋葵汁培养基的最优组合。结果显示,接种量、pH和柠檬酸三铵的浓度及葡萄糖的浓度为显著性影响因子,优化后的结果为:葡萄糖含量为0.99%,柠檬酸三铵含量为0.57%,pH为6.63,接种量为4.43%。优化后菌体的生长情况明显高于优化前的秋葵汁培养基中的生长,基本接近MRS培养基中的生长情况。
Factors significantly influenced on Lactobacillus acidophilus growth were selected by using the SPSS software after single factor experiment. Through the response surface design, optimum combination of okra juice medium of Lactobacillus acidophilus was determined. It showed that inoculation quantity, pH and the concentration of ammonium citrate and glucose were significant impact factors and the optimized result was that the glucose content 0.99%, ammonium citrate content 0.57%, pH 6.63, and inoculation quantity 4.43%. After optimization, the growth of bacteria in medium was obviously better than that of okra juice and it was basically close to the growth in MRS medium.
出处
《食品工业》
北大核心
2015年第7期146-150,共5页
The Food Industry
基金
郑州市科技创新团队项目(112PCXTD341)
郑州轻工业学院大学生科技活动项目
关键词
嗜酸乳杆菌
响应面
秋葵汁
Lactobacillus acidophilus
response surface methodology
okra juice
