摘要
以水曲柳(Fraxinus mandshurica Rupr.)不同时期的雌雄花为材料,应用实时荧光定量PCR技术,分析了18S rRNA、GAPDH、β-actin和TU 4个常用内参基因在水曲柳花中的表达情况。经Norm Finder软件分析选择出TU作为内参基因,并对水曲柳中的开花相关基因AG和SOC1在开花不同时期的表达情况进行了分析,结果表明:在水曲柳花发育期间AG、SOC1表达量在雌雄花间存在差异,AG基因在雌花初期表达量最高,之后逐渐降低,到了成熟胚囊时AG表达量又有所增加;而在雄花中AG在减数分裂时最高为1.53。SOC1基因在雌花中的表达量低于雄花。这一研究结果为深入了解水曲柳花发育过程中相关基因的表达提供理论依据。
We analyzed the temporal expressions of four housekeep genes, 18S rRNA, GAPDH, β-actin and TU, in ash（Fraxinus mandshurica Rupr. ） flowers with real-time quantitative PCR. With NormFinder software, TU could be selected as inner reference genes to study the expression of two genes, AG and SOC1, related to the flowering of ash. The expressions of both AG and SOC1 were different between male and female. The expression of AG started with a peak in female during the early period of flowering, followed by a decrease till mature embryo sac stage. In male, the expression of AG reached the peak （1.53） at meiosis stage. The expression of SOC1 in female was lower than that in male. Our results would provide evidence to study the expressions of flowering-related genes during the period of flower development in ash.
出处
《植物研究》
CAS
CSCD
北大核心
2015年第4期612-617,共6页
Bulletin of Botanical Research
基金
国家"十二五"支撑项目"水曲柳和白桦种子园1.5代种子园及优良繁殖材料规模化生产技术研究"(2012BAD21B0201-08)
国家基础科学人才培养基金(J1210053)
