摘要
History and the principle of laser capture microdissection （LCM）： LCM, also known as laser microdissection and pressure catapulting, is one of the most powerful and useful techniques in various research areas where isolation of heterogeneous cell population is required. The first use of laser as a cell operation method, was originated in early 1920s （Gilbrich-Wille, 2013）, and it became widely used as a microsurgical tool ill early 1960s. In the need of extremely small tissue isolation, laser beam of LCM has been modified and developed over several decades from Ultraviolet （UV） laser beam to high-energy nitrogen, infrared and carbon dioxide lasers （Gilbrich-Wille,
History and the principle of laser capture microdissection （LCM）： LCM, also known as laser microdissection and pressure catapulting, is one of the most powerful and useful techniques in various research areas where isolation of heterogeneous cell population is required. The first use of laser as a cell operation method, was originated in early 1920s （Gilbrich-Wille, 2013）, and it became widely used as a microsurgical tool ill early 1960s. In the need of extremely small tissue isolation, laser beam of LCM has been modified and developed over several decades from Ultraviolet （UV） laser beam to high-energy nitrogen, infrared and carbon dioxide lasers （Gilbrich-Wille,
