摘要
目的:探讨艾灸对大鼠脑缺血再灌注后内源性神经干细胞分化的影响,明确艾灸治疗脑缺血性卒中的作用机制,为艾灸治疗缺血性卒中提供新的理论依据。方法:选用Wistar大鼠72只,通过改良线栓法制备MCAO模型,将大鼠随机分为假手术组、模型组、艾灸组。每组分3天、7天、14天、21天4个时间点,每个时间点6只。通过腹腔注射Brdu来标记增殖的细胞。取大鼠"百会、太阳(双)"穴进行实验研究。通过TTC染色和HE染色来观察大鼠脑组织的病理学变化。应用免疫荧光技术,进行BrdU/GFAP、BrdU/NSE双标染色。通过显微图像分析系统对阳性结果进行分析。结果:艾灸组各时间点神经功能缺损评分与模型组比较,评分降低,具有显著性差异(P<0.05)。同一时间点艾灸组神经功能缺损评分与模型组比较有显著性差异(P<0.05);艾灸可促使脑缺血损伤后BrdU/GFAP、BrdU/NSE双标阳性细胞增加,于缺血损伤后3天达高峰。与模型组及假手术组比较有显著性差异(P<0.05)。结论:艾灸能显著促进脑缺血再灌注后大鼠神经功能恢复;艾灸能促进脑缺血再灌注后脑组织BrdU/GFAP双标阳性细胞、BrdU/NSE双标阳性细胞的表达,表明了艾灸能够促进神经干细胞向神经元细胞和神经胶质细胞分化。
Objective：To investigate the effect of moxibustion on endogenous neural stem cell differentiation in rats after cerebral ischemia reperfusion,to clarify the mechanism of action of moxibustion in the treatment of cer-ebral ischemic stroke, and to provide theoretical basis for moxibustion therapy in the treatment of ischemic＆amp;nbsp;stroke.Methods：72 Wistar rats were prepared by modified thread embolism of middle cerebral artery focal cere-bral ischemia reperfusion model （ MCAO model） ,and they were randomly divided into a sham operation group, a model group and a moxibustion group.Every group was subdivided into 3d, 7d, 14d, 21d 4 time points and at each time point there were 6 rats.The intraperitoneal injection of Brdu was used to label proliferating cells. The ratsˊ“Baihui, Taiyang（ double）” points were selected to conduct the experimental study.TTC staining and HE staining were used to understand brain ischemia and pathological brain tissue perfusion changes.Apply im-munofluorescence technique, BrdU/GFAP, BrdU/NSE double staining.Microscopic image analysis system was used to analyze the positive results.Results：In moxibustion group at each time point neural function defect score was compared with that of the model group and the score was decreased with a significant difference （ P 〈0.05 ） .At the same time point neural function defect score in the moxibustion group was compared with that in the model group,and there was a significant difference （ P〈0.05）;moxibustion could promote the increase of BrdU/GFAP, BrdU/NSE positive cells after cerebral ischemia injury, reaching the peak after 3 days.There was a significant difference compared with the model group and sham operation group （P〈0.05）.Conclusion：Moxibustion can significantly promote the recovery of neural function in rats with cerebral ischemia after reperfu-sion;moxibustion can promote the expression of brain tissue of cerebral ischemia and reperfusion BrdU/GFAP positive cells, BrdU/NSE positive cells, showing that moxibustion can promote neural stem cells into neurons and glial cell differentiation.
出处
《针灸临床杂志》
2015年第6期64-68,共5页
Journal of Clinical Acupuncture and Moxibustion
关键词
艾灸
脑缺血再灌注
神经干细胞
分化
实验研究
Moxibustion
Cerebral ischemia reperfusion
Neural stem cells
Differentiation
Experimental re-search
