摘要
目的观察蜈蚣提取液对局灶性脑缺血再灌注大鼠缺血半暗带组织形态和脑红蛋白(neuroglobin,NGB)表达的影响,从病理组织学水平和分子水平揭示蜈蚣防治脑缺血的作用机制。方法将大鼠随机分为假手术组,模型组,蜈蚣提取液低、中、高剂量组和益气活血方组,采用线栓法复制大脑中动脉局灶性脑缺血再灌注大鼠模型。模型复制后,分别给予相应的药物连续灌胃14d。光镜下观察各组大鼠额顶叶皮质缺血半暗带的组织形态学变化,采用免疫组织化学法检测NGB表达。结果模型组大鼠额顶叶皮质缺血半暗带水肿,神经细胞坏死明显,蜈蚣提取液低、中、高剂量组及益气活血方组大鼠额顶叶皮质缺血半暗带的病理变化较模型组显著改善。假手术组与模型组NGB的阳性表达面积和平均吸光度比较,差异无统计学意义(P<0.05)。蜈蚣提取液低、中、高剂量组NGB的阳性表达面积和平均吸光度与模型组比较,差异均有统计学意义(P<0.05),以蜈蚣提取液高剂量组的效应最显著。益气活血方组与低、中、高剂量蜈蚣提取液组NGB的阳性表达面积和平均吸光度比较,差异具有统计学意义(P<0.05),其效应与高剂量蜈蚣提取液相当(P>0.05)。结论蜈蚣提取液通过上调局灶性脑缺血再灌注大鼠缺血半暗带NGB表达,改善脑缺血再灌注造成的损伤,具有明显的剂量依赖性,高剂量蜈蚣提取液的效应与益气活血全方相当。
Objective To investigate the effects of centipede extract on the histomorphology and neuroglo- bin （NGB） expression in the ischemic penumbra after focal cerebral ischemia-reperfusion injury in rats and to explore the mechanism of action of centipede in preventing and treating cerebral ischemia at histopathological and molecular levels. Methods Rats were randomly divided into sham-operation group, model group,high-, middle-, and low-dose centipede extract groups, and qi-tonifying and blood-activating prescription （QBP） group. A rat model of focal cerebral ischemia-reperfusion of the middle cerebral artery was established by suture method. After modeling, these groups received respective treatments by gavage for 14 consecutive days. The histomorphology of ischemic penumbra in the frontal and parietal cortex was ob- served under a light microscope; the expression of NGB was measured by immunohistochemistry. Results The model group showed remarkable cerebral edema and neuronal necrosis in the ischemic penum- bra in the frontal and parietal cortex. By contrast, the high-, middle-, and low-dose centipede extract groups and QBP group had significantly reduced pathological changes in the ischemic penumbra in the frontal and parietal cortex. There were no significant differences in the positive expression area and mean optical density of NGB between the sham-operation group and model group （P〉0.05）. The positive expression area and mean opti- cal density of NGB showed significant differences between the model group and the high-, middle-, and low-dose centipede extract groups （P〈0.05）, and the difference was most significant between the model group and the high-dose centipede extract group. The positive expression area and mean optical density of NGB also showed sig- nificant differences between the QBP group and the high-, middle-, and low-dose centipede extract groups （P〈 0.05）, but the two indices showed no significant differences between the QBP group and the high-dose centipede extract group （P〉0.05）. Conclusion Centipede extract can reduce the focal cerebral ischemia-reperfusion injury in rats by up-regulating the expression of NOB in the ischemic penumbra in a significant dose-dependent manner, and high-dose centipede extract has comparable efficacy to QBP.
出处
《安徽中医药大学学报》
CAS
2015年第4期74-78,共5页
Journal of Anhui University of Chinese Medicine
基金
国家科技支撑计划项目(2012BAI26B01)
安徽省高等学校省级自然科学研究项目(KJ2012Z224)
安徽中医学院青年科研基金项目(2010qn001)
关键词
局灶性脑缺血再灌注
蜈蚣
益气活血方
组织形态学
脑红蛋白
focal cerebral ischemia-reperfusion injury
centipede
Qi-tonifying and blood-activating pre- seription
histomorphology） neuroglobin
