摘要
赤霉素(gibberellins,GAs)作为植物体内广泛存在的一类激素,可刺激植物叶和芽的生长,对植物的生长起着至关重要的调控作用。截至目前,已发现的GAs高达136种之多。所有已知的GAs均为二萜酸,具有相似的化学结构,都含有赤霉素烷骨架,即4个环,仅环上双键、羟基数目和位置不同。但由于GAs缺乏特定光学特性(紫外和荧光)和化学特性,同时在植物体内含量极低(一般为ng/g,甚至为pg/g水平),常规的分析方法和检测技术难以实现对该类物质的定性定量分析。近年来相关研究人员一直致力于各种高效、快速、灵敏的GAs分析新方法和新技术的研究。本文就GAs的检测方法和分析技术进行综述,为更好地开展赤霉素类植物激素的研究提供参考。
Gibberellins （GAs） are a class of phytohormones that exert profound and diverse effects on plant growth and development, such as seed germination and leaf expansion. Up to now, 136 members of GAs have been identified and recognized. All known GAs are diterpenoid acids with similar chemical structures, only double bonds, hydroxyl numbers and locations on gibberellin alkane skeleton are different. However, the content of GAs in plants is of ultra trace levels （ usually at ng/g and even pg/g levels） with little ultraviolet （UV） absorption, no fluo- rescence and no distinguishing chemical characteristics. Moreover, the matrix of plant samples is complicated. Thus, quantification of GAs is always extremely difficult. Nowadays, the bottle necks for the study of GAs in plants are due to the lack of efficient sample preparation and sen- sitive detection techniques. This article reviews the analytical methods for determination of GAs in recent years, hoping to provide some references to develop new methods and techniques.
出处
《色谱》
CAS
CSCD
北大核心
2015年第8期786-791,共6页
Chinese Journal of Chromatography
基金
国家自然科学基金项目(21305029
21477033)
高等学校博士学科点专项科研基金项目(20134103120002)
河南省高等学校青年骨干教师资助计划项目(2014GGJS-024)
关键词
赤霉素
植物激素
分析方法
样品前处理
综述
gibberellins
plant hormones
analytical methods
sample preparation
review
