摘要
以玉米品种‘垦玉6号’为材料,在150 mmol·L-1Na Cl胁迫条件下,研究葡萄糖(Glc)和蔗糖(Suc)浸种对玉米种子萌发阶段耐盐性的影响.结果表明:盐胁迫下,0.5mmol·L-1Glc、Suc浸种可促进玉米种子萌发及幼苗早期生长,其中Glc浸种玉米胚芽和胚根长及相应干质量增加到盐处理的1.5、1.3、2.1、1.8倍;Suc浸种玉米分别增加到1.7、1.3、2.7、1.9倍;盐胁迫下Glc、Suc浸种可减少胚芽中硫代巴比妥酸反应物(TBARS)和过氧化氢(H2O2)含量,与盐处理相比分别降低24.9%、20.6%;Glc、Suc浸种可显著提高盐胁迫下玉米胚芽超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、谷胱甘肽过氧化物酶(GPX)、谷胱甘肽还原酶(GR)的活性,并诱导葡萄糖6-磷酸脱氢酶(G6PDH)活性的升高,其中Glc浸种玉米SOD、APX、GPX、GR、G6PDH活性较盐处理分别提高66.2%、62.9%、32.0%、38.5%、50.5%,Suc浸种玉米较盐处理分别提高67.5%、59.8%、30.0%、38.5%、50.4%;Glc、Suc浸种胚芽中抗坏血酸(ASA)、谷胱甘肽(GSH)含量及ASA/DHA、GSH/GSSG显著提高,其中G6PDH活性与外源糖诱导的较强的抗氧化能力密切相关.Glc、Suc浸种还可提高盐胁迫下玉米胚芽中K+/Na+,分别为盐处理的2.3、2.4倍.外源Glc、Suc浸种可通过提高玉米种子抗氧化能力及维持体内K+和Na+离子平衡缓解盐胁迫对玉米种子萌发的抑制效应.
The maize variety Kenyu 6 was used to study the effects of exogenous glucose（ Glc） and sucrose（ Suc） on salt tolerance of maize seeds at germination stage under 150 mmol · L-1Na Cl treatment. Results showed that under salt stress condition,0. 5 mmol ·L-1exogenous Glc and Suc presoaking could promote seed germination and early seedling growth. Compared with the salt treatment,Glc presoaking increased the shoot length,radicle length and corresponding dry mass up to1.5,1. 3,2. 1 and 1. 8 times,and those of the Suc presoaking treatment increased up to 1. 7,1.3. 2.7 and 1.9 times,respectively. Exogenous Glc and Suc presoaking resulted in decreased levels of thiobarbituric acid reactive substances（ TBARS） and hydrogen peroxide（ H2O2） content of maize shoot under salt stress,which were lowered by 24. 9% and 20. 6% respectively. Exogenous Glc and Suc presoaking could increase the activities of superoxide dismutase（ SOD）,ascorbate peroxidase（ APX）,glutathione peroxidase（ GPX）,glutathione reductase（ GR） and induce glucose-6-phosphate dehydrogenase（ G6PDH） activity of maize shoot under salt stress. Compared with the salt treatment,Glc presoaking increased the activity of SOD,APX,GPX,GR and G6 PDH by66.2%,62.9%,32.0%,38.5% and 50.5%,and those of the Suc presoaking increased by 67.5%,59.8%,30. 0%,38. 5% and 50. 4%,respectively. Glc and Suc presoaking also significantly increased the contents of ascorbic acid（ ASA） and glutathione（ GSH）,ASA/DHA and GSH/GSSG.The G6 PDH activity was found closely related with the strong antioxidation capacity induced by exogenous sugars. In addition,Glc and Suc presoaking enhanced K＋/ Na＋in maize shoot by 1.3 and1.4 times of water soaking salt treatment,respectively. These results indicated that exogenous Glc and Suc presoaking could improve antioxidation capacity of maize seeds and maintain the in vivo K＋/ Na＋ion balance to alleviate the inhibitory effect of salt stress on maize seed germination.
出处
《应用生态学报》
CAS
CSCD
北大核心
2015年第9期2735-2742,共8页
Chinese Journal of Applied Ecology
基金
国家科技支撑计划项目(2013BAD07B01-05)
教育部博士学科点博导类专项科研基金项目(20122305110001)
"十二五"农村领域国家科技计划项目(2011BAD16B1103)
黑龙江省自然科学基金项目(C201324)
黑龙江省应用技术研究与开发计划项目(GZ13B018)
大庆市科学技术项目(SJH-2013-35)资助
关键词
盐胁迫
外源糖
种子萌发
抗氧化能力
离子平衡
salt stress
exogenous sugar
seed germination
antioxidation capacity
ion balance
