摘要
目的：评价Ro20-1724对氯胺酮重复麻醉致幼龄大鼠认知功能障碍的影响。方法健康SD大鼠32只，21日龄，雌雄不拘，体重45～55 g，采用随机数字表法分为4组（ n＝8）：对照组（ C组）、氯胺酮组（ K组）、氯胺酮＋ Ro20-1724组（ K＋R组）和氯胺酮＋无水乙醇组（ K＋E组）。 K组、K＋R组和K＋E组腹腔注射70 mg∕kg氯胺酮，1次∕d，连续7 d；C组腹腔注射等容量生理盐水。正常饲养2 d后，K＋R组、K＋E组分别腹腔注射Ro20-17240．5 mg∕kg或等容量无水乙醇，K组和C组腹腔注射等容量生理盐水，1次∕d，连续7 d。采用Morris水迷宫实验测定认知功能，记录逃避潜伏期和穿越原平台位置次数。行为学实验结束当日处死大鼠，取海马组织，采用ELISA法测定CA1区脑源性神经营养因子（ BDNF）含量。结果与C组比较，K组和K＋E组第1～4天时逃避潜伏期延长，K＋R组第3，4天时逃避潜伏期延长，K组、K＋R组和K＋E组穿越原平台位置次数减少，海马CA1区BDNF含量降低（ P＜0．05或0．01）；与K组比较，K＋R组第3，4天时逃避潜伏期缩短，穿越原平台位置次数增多，海马CA1区BDNF含量升高（P＜0．05或0．01），K＋E 组上述各指标差异无统计学意义（P＞0．05）。结论 Ro20-1724可改善氯胺酮重复麻醉致幼龄大鼠认知功能障碍，其机制与促进 BDNF 的合成有关。
Objective To evaluate the effects of Ro20-1724 on cognitive dysfunction induced by repetitive ketamine anesthesia in immature rats. Methods Thirty-two Sprague-Dawley rats of both sex, aged 21 days, weighing 45-55 g, were randomly divided into 4 groups （ n=8 each ） using a random number table： control group （ group C ） , ketamine group （ group K ） , ketamine＋Ro20-1724 group （ group K＋R） , and ketamine＋anhydrous alcohol group （ group K＋A） . In K, K＋R and K＋E groups, 70 mg∕kg ketamine was intraperitoneally injected once a day for 7 consecutive days. The equal volume of normal saline was given instead in group C. Two days after the rats were fed a common diet, 0.5 mg∕kg Ro20-1724 and the equal volume of anhydrous alcohol were injected in K＋R and K＋E groups, respectively, and the equal volume of normal saline was given instead in K and C groups, once a day for 7 consecutive days. Morris water maze test was used to test cognitive function, and the escape latency and frequency of crossing the original platform were recorded. The rats were sacrificed after the end of behavior tests, and hippocampi were removed to detect the content of brain-derived neurotrophic factor （ BDNF） in CA1 region using enzyme-linked immunosorbent assay. Results Compared with group C, the escape latency was significantly prolonged on 1st-4th days in K and K＋E groups, the escape latency was prolonged on 3rd-4th days in K＋R group, and the frequency of crossing the original platform and content of BDNF in CA1 region were decreased in K, K＋R and K＋E groups. Compared with K group, the escape latency was significantly shortened, and the frequency of crossing the original platform was increased on 3rd-4th days, and the content of BDNF in CA1 region was increased in K＋R group, and no significant changes were found in the parameters mentioned above in K＋E group. Conclusion Ro20-1724 can improve cognitive dysfunction induced by repetitive ketamine anesthesia in immature rats, and enhanced production of BDNF is involved in the mechanism.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2015年第5期543-545,共3页
Chinese Journal of Anesthesiology
基金
国家自然科学基金（81000469）
江苏省卫生厅科研项目（H201070）
关键词
磷酸二酯酶抑制剂
氯胺酮
认知障碍
儿童
Phosphodiesterase inhibitors
Ketamine
Cognition disorders
Child
