摘要
目的建立HPLC-ELSD法同时测定酸枣仁合欢方（SHF）提取物中酸枣仁皂苷A和B含量测定方法。方法色谱柱：ODS HYPERSIL C18（4.6 mm×250 mm,5μm）,流动相：乙腈∶水（32∶68）,体积流速：1.0 ml·min^-1,柱温：35℃,蒸发光散射检测器漂移管温度：102℃,载气流速：2.8 L·min^-1。结果酸枣仁皂苷A和酸枣仁皂苷B分别在0.60-3.30μg（r=0.9997）、0.90-4.50μg（r=0.9993）范围内线性关系良好,平均回收率分别为100.1%（RSD=1.48%）、100.3%（RSD=1.15%）。结论该法操作简便,结果准确,专属性强,能够用于酸枣仁合欢方提取物及其制剂的质量控制。
Objective To establish an HPLC-ELSD method for simultaneous determination of jujuboside A and jujuboside B in Suanzaoren-hehuan formula（ SHF） extracts. Methods ODS HYPERSIL C18column（ 4. 6 mm × 250 mm,5μm） was adopted,the mobile phase was acetonitrile-water（ 32 ∶ 68）,the flow rate was 1ml · min^-1,column temperature： 35℃,the evaporator tube temperature of ELSD was set at 102℃,the nebulizing gas flow-rate was 2. 8 L·min^-1. Results The linear ranges of jujuboside A and jujuboside B were 0. 60 - 3. 30μg（ r = 0. 9997）,0. 90 ~ 4. 50μg（ r = 0. 9993）,respectively. The average recoveries were100. 1%（ RSD = 1. 48%）,100. 3%（ RSD = 1. 15%）,respectively. Conclusion The method was simple and accurate,and could be used as a method for the quality control of Suanzaoren-hehuan formula extracts.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2015年第8期1796-1798,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.81173530)
天津市自然科学基金重点项目(No.12JCZDJC25900)
