摘要
目的建立以邻苯二甲醛为衍生化试剂的柱前衍生化高效液相色谱法（HPLC），探究普鲁士蓝对铊（T1）中毒犬不同脑区兴奋性氨基酸（EAA）和抑制性氨基酸（IAA）神经递质含量的影响。方法18只比格犬随机分为对照组（NS组）、染毒对照组（T1组）和染毒普鲁士蓝治疗组（PB组），T1组及PB组予以硝酸亚铊（TINO3）半数致死剂量（LD50=45mg／kg）灌胃染毒，PB组予以普鲁士蓝治疗，10d后处死比格犬分离脑组织，采用HPLC测定不同脑区EAA及IAA递质的含量。结果T1中毒犬部分脑区EAA含量升高，普鲁士蓝治疗可降低这一变化，各脑区IAA变化不明显。结论本实验建立的方法能同时测定犬脑组织中天冬氨酸（Asp）、谷氨酸（Gh）、甘氨酸（Gly）和y-氨基丁酸（GABA）的含量，方法稳定、精确；T1中毒后增强了EAA的释放，导致兴奋性毒性。
Objective To establish a method as o -Phthalaldehyde as derivative reagent pre - column derivatization by high performance liquid chromatography （HPLC）. Explore the impact of Prussian Blue on excitatory amino acids （EAA） and inhibitory amino acids （IAA） neurotransmitter content from different brain regions of thallium poisoning dogs. Methods Eighteen beagle dogs were randomly divided into a control group （ NS group）, exposure control group （ TI group） and exposure therapy group by Prussian Blue （PB group）. TI group and the PB group were given thallium nitrate （T1NO3 ） median lethal dose （LD50 = 45 mg / kg） by gavage. Dogs in PB group were treated by Prussian Blue, and put to death and dessect brain tissue after 10 days. EAA and IAA content in different brain regions was analyzed by HPLC. Results The EAA of thallium poisoning dogs increased in part of brain areas, and Prussian blue treatment can reduce this change, but IAA changed obviously in all brain areas. Conclusion The method can evaluate the content of Aspartate （Asp）, Glutamate （Glu）, Glycine （Gly）, y- aminobutyric acid （GABA） in brain tissue of dogs at the same time stably and accurately. Thallium poisoning can enhance the release of EAA and cause excitotoxicity.
出处
《中国急救医学》
CAS
CSCD
北大核心
2015年第9期854-858,共5页
Chinese Journal of Critical Care Medicine
基金
国家新药创制重大项目（2011zxJ09102）
