摘要
对木聚糖酶嗜热真菌嗜热踝节菌F1208的产酶条件进行优化，经单因素试验及Box—Behnken响应面分析，确定该菌液体发酵培养基，其组成是：碳源为玉米芯粉，碳源粒度24—40目，碳源质量分数5％；氮源为大豆蛋白胨与酵母膏复合氮源（质量比l：3），氮源质量分数2％；辅助添加物甘油（0．2％）；培养基初始pH6．4。优化后最佳产酶条件是：装液量60mL，培养温度48℃，摇床转速200r／min。在此条件下发酵培养7d，产酶量迭3026．65U，mL，是初始酶活（960．39U／mL）的3．15倍，为同类研究中较高水平，具有较好的应用前景。
The culture conditions of a thermophilic fungus F1208 with high yield of xylanase activity were optimizedby single-factor experiment and Box-Behnken design matrix of response surface methodology. The influences of medium composition and fermentation conditions in the cultivation were investigated. A high xylanase activity of 3 026.65 U/mL was obtained in 7 days, which was 3.15 fold of the initial enzyme activity （960.39 U/mL）. The results indicated optimum conditions were corncob, mesh number 24-40, 5%; soya peptone and yeast cream （ratio 1：3） 2%; glycerine 0.2%; pH 6.4, and the fermentation conditions was 60 mL, 48℃ and 200 r/min.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2015年第8期85-93,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然基金-青年科学基金项目(No.31201449)
国家高技术研究发展(863)计划项目(2012AA021502)
关键词
木聚糖酶
嗜热踝节菌
响应面分析
优化
xylanase
Talaromyces thermophilus
Box-Behnken design
optimization
