摘要
目的：建立HPLC法同时测定了哥王中芦荟大黄素和大黄素甲醚含量的方法。方法：采用C18色谱柱（4.6 mm×250 mm,5μm）,流动相A为乙腈,流动相B为体积分数为0.15%磷酸水,梯度洗脱,流速1.0 m L·min^-1,检测波长254 nm,柱温30℃。结果：芦荟大黄素和大黄素甲醚的质量浓度分别在1.584-25.34（r=0.999 2）和1.598-25.57μg·m L^-1（r=0.999 3）内与峰面积呈良好的线性关系,平均回收率分别为100.2%（RSD 1.8%）和99.7%（RSD 1.1%）。不同产地了哥王中大黄素甲醚和芦荟大黄素含量差异较大,其中广东韶关产地的芦荟大黄素含量最高,大黄素甲醚含量最低;福建福州产地的大黄素甲醚含量最高,芦荟大黄素含量最低;浙江（批号120724）了哥王未检测到芦荟大黄素,贵州安顺产地的了哥王中未检测到大黄素甲醚。结论：该方法简便、快速、准确,为了哥王药材的质量控制提供参考。
Objective： To develop an HPLC method for the simultaneous determination of aloe-emodin and physeion in Wikstroemia indica. Method： Diamonsil Clscolumn （4. 6 mm ×250 mm, 5 μm） was eluted with the mobile phases of acetonitrile-0. 10% phosphoric acid water in a gradient mode. The UV detection wavelength was 254 nm. The flow rate was 1.0 mL ·min^-1 The column temperature was set at 30 ℃ , and the sample size was 20 μL. The difference in the content of aloe-emodin and physcion in W. indica from from ten origins was compared. Result： All of aloe-emodin and physcion samples showed a good linearity within the range of 1. 584-25.34 mg· L^-1 （r = 0. 999 2）, 1. 598-25.57 mg ·L^-1 （r = 0. 999 3）, respectively. The average recoveries were 100. 2% and 99.7% , with RSD of 1.8% and 1.1% , respectively. There was a great difference in the content of aloe-emodin and physeion in W. indica of different origins. Specifically, W. indica from Shaoguang, Guangdong province, had the highest content of aloe-emodin but the lowest content of physcion; W. indica from Fuzhou, Fujian province, had the highest content of physeion but the lowest content of aloe-emodin. Aloe-emodin was not detected in 120724 W. indica from Zhejiang, and physcion was not detected from Anshun, Guizhou province. Conclusion： The method is simple, rapid and accurate and so can be applied for the quality control of W. indica.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第18期73-75,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
辽宁省教育厅创新团队项目(LT2012018)
辽宁省科技厅项目(2011412004)
