摘要
为探讨鸡骨草总黄酮的超声波提取条件及清除自由基的能力,采用单因素试验和正交试验优化鸡骨草总黄酮的超声波提取工艺,分别采用水杨酸、邻苯三酚自氧化法测定鸡骨草总黄酮提取液对羟自由基、超氧离子自由基的清除作用。结果表明,鸡骨草总黄酮的超声波提取最优工艺是用25倍70%乙醇浸泡鸡骨草粉末,超声波提取4次,每次18 min,所得提取液总黄酮提取率为1.75%;鸡骨草提取液浓度越高,对羟自由基和超氧阴离子的清除作用越大。超声波提取法适用于鸡骨草总黄酮的提取,且提取液对羟自由基和超氧离子自由基具有一定的清除作用。
The conditions of ultrasonic extraction of total flavonoids in Abrus cantoniensis L. and its ability of free radical scavenging were investigated. The optimized condition of extraction of total flavonoids was examined through single factor test and orthogonal design. The autoxidation of salicylic acid and pyrogallol was used to assay the abilities of the extract of clearing the free radical of ·OH and ·O2^-. The most optimal extracting process was ultrasonic extraction four times with 25 times of 70% ethanol by 18 minutes each time.Under this condition, the amount of the total flavonoids of the extract was1.75%. And the abilities of the extract of clearing ·OH and ·O2^-enhances with the increase of concentration. it is indicated that the method of ultrasonic extraction could be used to determine the total flavonoids in Abrus cantoniensis L.. And the extract of Abrus cantoniensis L. could effectively clear the free radical of ·OH and ·O2^-.
出处
《湖北农业科学》
2015年第14期3502-3505,共4页
Hubei Agricultural Sciences
基金
广东高校特色调味品工程技术开发中心项目(GCZX-B1103)
关键词
鸡骨草
总黄酮
超声波
自由基
Abrus cantoniensis L.
total flavonoids
ultrasonic extraction
free radical
