摘要
为了解闽西地区2013年以来猪伪狂犬病毒（PRV）流行株的现状、分子生物学特征和遗传演化规律,本试验收集了闽西不同地区的14株PRV分离株,并对其TK基因进行遗传变异分析。结果表明：14株PRV分离株的TK基因与其他参考毒株相应序列具有严格的保守性,这些毒株之间的核苷酸和氨基酸序列同源性分别为98.3%~99.8%和96.9%~99.4%。氨基酸多序列比对发现,14株PRV分离株在2个位点发生了独特性的氨基酸突变,即第16位氨基酸（除PRV FJLY04和FJLY06株外）由K突变为R和第129位氨基酸由S突变为G,这是国内首次报道TK基因在该位置发生氨基酸突变的毒株。其中,第16位氨基酸突变导致TK蛋白ATP结合结构域由-G＊＊G＊GK-转变为-G＊＊G＊GR-,同时该位点还位于TK蛋白的抗原表位区内。这些新的氨基酸突变与PRV致病性及其与生物学特性的关系有待进一步研究。
To study the genetic diversity, the molecular epidemiology and phylogenetic relationship of porcine pseudorabies virus （PRV） in western Fujian province,the TK gene in 14 PRV isolates from 2013 to 2014 were sequenced and analyzed. The results showed that the TK gene sequence Of 14 PRV isolates shared a strict conservative with the reference PRV strains,and the homologies of the TK gene nucleotide sequences and amino acid sequences were 98.3% to 99.8%and 96.9% to 99.4% ,respectively. The alignment results revealed that 2 unique amino acid mutations were iden- tified in the TK sequence of 14 PRV isolates compared to the reference strains. It was that Lys mutated into Arg at position aa 16 （except the PRV FJLY04 and FJLY06 strains） and Ser mutated into Gly at aa 129,respectively,which is the first report about the mutations in these postions of TK in China. Among them,the amino acid mutation at position aa 16 led to ATP-binding domain ofTKconsistedof-G ＊＊G＊GK-into-G ＊＊G＊ GR- , and at the same time , these sites were al- so located in the antigenic epitope regions of TK protein. Our data would be helpful to understand- ing the evolutionary characteristics of PRV.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2015年第9期1415-1421,共7页
Chinese Journal of Veterinary Science
基金
福建省农业科技重大专项资助项目(2014NZ01060015)
福建省教育厅资助省属高校项目(JK2013052)
关键词
猪伪狂犬病毒
TK基因
突变
porcine pseudorabies virus
TK gene
mutation
