摘要
目的:探讨microRNA-21在口腔鳞状细胞癌中的表达情况,以及与预后关系。方法:选取98例口腔鳞状细胞癌患者组织及正常黏膜组织标本,同时,选取50例非肿瘤患者黏膜组织,分别提取标本中总RNA,利用RT-PCR检测microRNA-21在口腔鳞状细胞癌及正常黏膜组织中相对表达量,探讨其与临床病理特征的关系,利用Kaplan-Meier法分析其与预后的关系。结果:microRNA-21在口腔鳞状细胞癌组织中相对表达量(2.37±1.01),显著高于正常黏膜组织中的(0.97±0.08)和非肿瘤患者黏膜组织中的(0.94±0.07),差异具有统计学意义(P<0.05),;microRNA-21表达与患者性别、年龄、肿瘤位置、肿瘤大小和TNM分期无关,而与分化程度和是否发生淋巴结转移有关(P<0.05);将microRNA-21相对表达量的中位值2.41为界,将所有患者分为高表达组和低表达组,高表达组患者中位生存期38个月,低表达组患者中位生存期44个月,差异具有统计学意义(P<0.05)。结论:microRNA-21在口腔鳞状细胞癌中呈高表达,且与分化程度和淋巴结转移有关,并可影响患者预后。
Objective： To investigate the expression of microRNA--21 in oral squamous cell carcinoma and its correlation with prognosis. Methods： 98 cases of oral squamous cell carcinoma tissue and normal tissue samples were selected. Meanwhile, mucosa of 50 cases of non--cancer patients was selected, Total RNA was isolated from specimens, respectively. The relative expression levels of microRNA-21 in oral squamous cell carcinoma and normal mucosa were detected by RT-PCR. Its correlations with clinicopathological features and prognosis were analyzed by Kaplan-Meier method. Results： The relative expression level of microRNA--21 in oral squamous cell carcinoma （2.37± 1.01） was significantly higher （P±0.05） than normal mucosa （0.97±0.08） and non--cancer patients mucosa （0. 94±0.07）. The expression of microRNA-21 was not correlated with sex, age, tumor location, tumor size and TNM stage, but significantly correlated with the degree of differentiation and lymph node metastasis （P±0. 05）. All patients were divided into high and low expression groups according to the median value of microRNA-21 relative expression （2.41）. The median survival of patients was 38 months in high expression group, while 44 months in low expression group, and the difference was statistically significant （P〈0. 05）. Conclusion： The expression level of microRNA-21 was elevated in oral squamous cell carcinoma, which was correlated with the degree of differentiation and lymph node metastasis, and affected the prognosis of patients.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2015年第9期927-930,共4页
Journal of Oral Science Research
