摘要
目的研究急性脑梗死发生肺部感染患者病原菌分布及对氧化应激反应蛋白表达的影响,为临床治疗提供参考依据。方法对2011年4月-2014年4月医院诊治的110例急性脑梗死患者肺部感染病原菌进行分离、培养和鉴定,检测患者血清中丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酯(CAT)和谷胱甘肽过氧化物酶(GSHpx)水平,ELISA法分析血清中NADPH氧化酶亚基的表达。结果 110例急性脑梗死发生肺部感染患者共分离出病原菌396株,其中革兰阳性菌占55.05%,革兰阴性菌占44.95%;患者血清中MDA及p22、p47、p67亚基和gp91亚基表达明显升高,而SOD、CAT和GSHpx水平明显降低。结论急性脑梗死发生肺部感染患者病原菌中,革兰阳性菌和革兰阴性菌比例相当,且感染能诱导患者氧化应激蛋白水平升高。
OBJECTIVE To explore the distribution of pathogens causing pulmonary infections in the patients with acute cerebral infarction and observe the impact on expression of oxidative stress response protein so as to provide guidance for clinical treatment .METHODS A total of 110 acute cerebral infarction patients complicated with pul‐monary infections who were treated in the hospital from Apr 2011 to Apr 2014 were enrolled in the study ,the pathogens were isolated ,cultured ,and identified .The levels of serum malondialdehyde （MDA） ,superoxide dis‐mutase （SOD） ,catalase ester （CAT） ,and glutathione peroxidase （GSHpx） were measured;the expression of se‐rum NADPH oxidase subunit was analyzed by using ELISA .RESULTS Totally 396 strains of pathogens were iso‐lated from the 110 acute cerebral infarction patients complicated with pulmonary infections ,of which 55 .05% were gram‐positive bacteria ,and 44 .95% were gram‐negative bacteria .The levels of serum MDA ,subunit p22 ,p47 , p67 ,and subunit gp91 were significantly elevated ,however ,the levels of SOD ,CAT ,and GSHpx were remark‐ably reduced .CONCLUSION Among the pathogens isolated from the acute cerebral infarction patients complicated with pulmonary infections ,the proportion of the gram‐positive bacteria is the same as the proportion of the gram‐negative bacteria ,and the infections may induce the elevated level of oxidative stress response protein .
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2015年第20期4600-4601,4615,共3页
Chinese Journal of Nosocomiology
基金
贵州省科技计划基金资助项目(201404)
关键词
急性脑梗死
肺部感染
病原菌
氧化应激反应
Acute cerebral infarction
Pulmonary infection
Pathogen
Oxidative stress response protein
