摘要
目的观察复肾功方治疗慢性肾功能衰竭的可能作用机制。方法将55只SD大鼠随机分为正常组、模型组及复肾功方低、中、高剂量组,每组11只。正常组自由饮食,其余4组大鼠食用含0.5%腺嘌呤饲料制造慢性肾功能衰竭模型。造模后正常组和模型组给予生理盐水20 ml/kg灌胃;复肾功方低、中、高剂量组分别以4、8、16 g/kg灌胃复肾功方,每日1次,连续30天。检测各组大鼠血肌酐(SCr)和尿素氮(BUN)水平,HE染色检测肾组织形态学改变,免疫组化检测音猬因子(Shh)、神经胶质瘤相关癌基因同源蛋白1(Gli1)的表达。结果与正常组比较,模型组大鼠SCr和BUN水平及肾组织Shh、Gli1水平明显升高(P<0.05),肾间质出现明显纤维化病变。复肾功方各剂量组大鼠SCr和BUN水平及肾组织Shh、Gli1水平较模型组均明显降低,其中复肾功方高剂量组各指标水平最低,3组间两两比较差异有统计学意义(P<0.05)。结论复肾功方能明显改善慢性肾功能衰竭大鼠肾功能,且随剂量增加改善效果更好,其机制可能与抑制Shh信号通路的激活有关。
Objective To observe the possible function mechanism of Fushengong Formula in treating chronic renal failure. Methods Fifty-five Sprague Dawley rats were randomly divided into normal group,model group and low,middle and high Fushengong Formula groups,with 11 rats in each. Normal group ate freely. Other 4 groups of rats were fed with fodder containing 0. 5% adenine to make chronic renal failure model. The normal and model groups were orally gavaged with normal saline 20 ml / kg after modeling; while the low,middle and high Fushengong Formula groups were orally gavaged with 4,8,or 16 g / kg of Fushengong Formula respectively,once per day,for 30 days.Serum levels of creatinine（ SCr） and urea nitrogen（ BUN） of rats in each group were tested. Kidney tissue morphology change was observed through HE dyeing. Expression levels of sonic hedgehog（ Shh） and glioma related cancer gene homologous proteins 1（ Gli1） were tested immunohistochemically. Results Serum levels of SCr and BUN and expression levels of Shh and Gli1 in renal interstitium of rats increased remarkably（ P〈0. 05）,and there appeared obvious fibrotic lesions in renal interstitium in model group compared with those in normal group. Serum levels of SCr and BUN and expression levels of Shh and Gli1 of rats in each dose of Fushengong Formula group decreased remarkably compared with that in model group（ P〈0. 05）,especially in high-dose Fushengong Formula group. There was statistically significant differences in those indexes between each two Fushengong Formula groups（ P〈0. 05）.Conclusion Fushengong Formula could remarkably improve renal function of rats with chronic renal failure. And the improving effects were better when dose increased. Its possible mechanism may have a relationship with inhibiting activating Shh signal pathway.
出处
《中医杂志》
CSCD
北大核心
2015年第20期1771-1774,共4页
Journal of Traditional Chinese Medicine
基金
重庆市自然科学基金(cstc2011jj A10058)
重庆市九龙坡区社会事业类科技项目(九龙坡科委发[2011]62号
九龙坡科委发[2013]37号)
