摘要
目的：建立同时测定复方苯海拉明搽剂中樟脑和薄荷脑含量的方法。方法：采用气相色谱法。色谱柱为Agilent19095N-123 INNOWAX毛细管柱,柱温为120℃,进样口温度为220℃,检测器为氢火焰离子化检测器,检测器温度为260℃,载气为氮气,柱流速为6.0 ml/min,空气流速为450 ml/min,氮气流速为40 ml/min,分流比为20∶1,进样量为1μl。结果：樟脑和薄荷脑质量浓度线性范围为0.612～6.12、0.593～5.93 mg/ml（r=0.999 9、0.999 9）;精密度、稳定性、重复性试验的RSD〈2%;加样回收率分别为98.0%～100.7%、98.7%～100.7%,RSD分别为1.0%、0.7%（n=6）。结论：该方法操作简便、结果准确、重复性好,可用于测定复方苯海拉明搽剂中樟脑和薄荷脑的含量。
OBJECTIVE： To establish a method for simultaneous determination of camphor and menthol in Compound diphen- hydramine liniment. METHODS： GC was performed on the column of Agilent 19095N-123 INNOWAX capillary column, column temperature was 120 %, injection volume temperature was 220 ℃, FID detector was used with the temperature of 260 ℃, carrier gas was nitrogen gas, flow of column was 6.0 ml/min, air was 450 ml/min, hydrogen gas was 40 ml/min, split ratio was 20：1 and injection volume was 1 μl. RESULTS： The linear range of mass concentration was 0.612-6.12 mg/ml （r=0.999 9） for camphor and 0.593-5.93 mg/ml （r=0.999 9） for menthol; RSDs of precision, stability and reproducibility tests were lower than 2% ; the recov- eries were respectively 98.0%-100.7% （RSD=1.0%, n=6） and 98.7%-100.7% （RSD=0.7%, n=6）. CONCLUSIONS： The meth- od is simple, accurate and reproducible, and can be used for the contents determination of camphor and menthol in Compound di- phenhydramine liniment.
出处
《中国药房》
CAS
北大核心
2015年第30期4297-4299,共3页
China Pharmacy
