摘要
背景:国内外对静磁场加载许旺细胞的研究较少,对其产生的生物学效应尚不清楚。目的:探索静磁场对许旺细胞分泌神经生长因子水平的影响。方法:将传代良好的许旺细胞随机分为3组,分别为0.05 m T静磁场组、0.1 m T静磁场组、空白对照组。从接种第2天即开始静磁场加载,每天曝磁12 h,空白对照组不进行静磁场加载。加载6 d后利用RT-PCR技术检测许旺细胞中神经生长因子m RNA的表达,ELISA技术检测许旺细胞分泌神经生长因子水平。结果与结论:静磁场组培养上清中神经生长因子m RNA的表达和分泌神经生长因子水平显著高于空白对照组(P<0.05);0.1 m T静磁场组神经生长因子m RNA的表达和分泌神经生长因子水平虽高于0.05 m T静磁场组,但是差异无显著性意义(P>0.05)。结果表明一定强度的静磁场可以促进许旺细胞快速分泌神经生长因子。
BACKGROUND： There are less studies on static magnetic field, and its biological effects are still unclear. OBJECTIVE： To investigate the static magnetic field effect on the secretion of nerve growth factor from Schwann cells. METHODS： Schwann cells were randomly assigned into three groups： 0.05 m T sciatic magnetic field group, 0.1 m T sciatic magnetic field group and control group. At 2 days of inoculation, the cells were exposed to the sciatic magnetic field, 12 hours per day. Cells in the control group were not exposed to the sciatic magnetic field. After 6 days of exposure, RT-PCR method was used to detect nerve growth factor m RNA expression in Schwann cells, and ELISA was adopted to detect the level of nerve growth factor secreted from Schwann cells. RESULTS AND CONCLUSION： The m RNA expression and secreted level of nerve growth factors in the culture supernatant were significantly higher in the two sciatic magnetic field groups than the control group（P〈0.05）, especially in the 0.1 m T group. However, there was no difference between the 0.05 m T and 0.1 m T groups（P〉0.05）. These findings indicate that the static magnetic field with certain intensity can promote the secretion of nerve growth factors from Schwann cells.
出处
《中国组织工程研究》
CAS
北大核心
2015年第36期5848-5852,共5页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金项目(81460198)
项目名称:EZH2基因对神经干细胞修复脊髓损伤的表观调控作用及机制研究
广西卫生和计划生育委员会自筹经费科研课题(Z2015400)
课题名称:静磁场对血旺细胞体外扩增的影响~~
关键词
干细胞
培养
静磁场
国家自然科学基金
Schwann Cells
Nerve Growth Factor
Electromagnetic Fields
