摘要
目的：探讨不同梅毒检测方法用于妊娠期女性梅毒筛查的临床价值,寻找最为适合的筛查方法。方法：选取妊娠期女性1529例作为研究对象,采用梅毒特异性抗体检测法判断孕妇是否感染梅毒,并据此分为感染梅毒的观察组（n=14）和未感染梅毒的对照组（n=1515）。分别采用TRUST（甲苯胺红不加热血清试验）、ELISA（酶联免疫吸附试验）、TPPA（梅毒螺旋体明胶凝集试验）进行检测,并对结果进行比较和分析。结果：TRUST、ELISA、TPPA敏感性分别为64.29%、85.71%、100.00%,阳性预测值分别为56.25%、85.71%和100.00%,TPPA〉ELISA〉TRUST,三种检测方法差别有统计学意义（P〈0.05）。TPPA敏感性和特异性均高达100%,其阳性患者14例标本滴度为1∶16~1∶128,其余患者标本滴度均≤1：4。通过pearson直线相关性分析,TRUST法滴度与ELISA法A值之间没有明显的相关性（r=0.391,P〉0.05）;TRUSR法与TPPA法滴度具有明显的相关性（r=0.927,P〈0.05）。结论：与TRUST相比ELISA的敏感性更高,可用于妊娠期梅毒筛查,而TRUST滴度可用于梅毒疗效监测。
Objectives： To investigate the clinical value of different methods to detect syphilis for pregnant women,so as to find the most appropriate screening method. Methods： 1529 pregnant women were selected as research subjects. A specific antibody detection of syphilis in pregnant women was used to determine whether they were infected with syphilis. According to the results,they were divided into observation group（ n = 14） and control group（ n = 1515）. TRUST（ toluidine red unheated serum test）,ELISA（ enzyme- linked immunosorbent assay）,TPPA（ Treponema pallidum wise teaching agglutination test） were used and the results of the two groups were compared and analyzed. Results： Sensitivity of TRUST,ELISA and TPPA were 64. 29%,85. 71% and 100. 00%respectively and positive predictive values were 56. 25%,85. 71% and 100. 00% respectively,TPPA ELISA TRUST. Differences among three detection methods were statistically significant（ P〈0. 05）. TPPA sensitivity and specificity were both 100%,titer of 14 patients whose specimens were positive was 1 ∶ 16 to 1 ∶ 128,and the other patient specimens＇ titers were ≤1： 4. By Pearson linear correlation analysis,there was no clear correlation between titer of ELISA method and A value of TRUST（ r = 0. 391,P〈0. 05）; TRUSR method and TPPA titer method had significant correlation（ r = 0. 927,P〈0. 05）. Conclusion： Compared with TRUST,ELISA has higher sensitivity,which can be used for syphilis screening during pregnancy,and TRUST titer can be used to monitor the efficacy of syphilis treatment.
出处
《中国性科学》
2015年第10期55-57,共3页
Chinese Journal of Human Sexuality
关键词
梅毒
孕妇
筛查
Syphilis
Pregnant women
Screening
