摘要
目的建立巴戟天药材中耐斯糖含量的近红外光谱测定方法。方法用高效液相色谱法测定114批巴戟天药材中耐斯糖含量,采集近红外光谱后,运用多元散射校正法,结合最小偏二乘法建立巴戟天耐斯糖含量的定量模型。结果建立的耐斯糖近红外光谱定量模型,内部交叉验证决定系数为0.979 1,校正标准偏差为0.909 0,预测标准差为0.909 0,交叉验证的标准偏差为1.093 0。结论该含量测定近红外光谱模型稳定、准确适用于巴戟天药材中耐斯糖的含量测定。
Objective： To establish the quantitative determination model of nystose in Morinda officinalis by Near Infrared Spectroscopy（NIRS）. Methods： The nystose contents of 144 samples were Mmeasured by HPLC,and their spectrum were collected. Combined with MSC and PLS,the quantitative determination model were established. Results： The determination coefficient,root-mean-square error of cross-validation,root-mean-square error of calibration and root-mean square error of prediction were 0.9791,0.9090,0.9090 and 1.0930 respectively in the created calibration model. Conclusion： The quantitative determination model is steady,and can determine the content rapidly and accurately.
出处
《井冈山大学学报（自然科学版）》
2015年第5期94-99,共6页
Journal of Jinggangshan University (Natural Science)
基金
广东省科技计划项目(2009B030801044)
关键词
巴戟天
耐斯糖
近红外光谱
定量模型
Morinda officinalis
nystose
NIRS
quantitative determination model
