摘要
目的观察蜂胶黄酮对氧化型低密度脂蛋白(ox-LDL)诱导的炎症反应单核细胞中核苷酸结合寡聚化结构域样受体3(NLRP3)炎性小体活性的影响。方法培养单核细胞THP1并分为5个组。实验1、2、3组及模型组加入ox-LDL 100μg/m L制作单核细胞炎症反应模型;实验1、2、3组在加入ox-LDL前分别给予5、10、15μg/m L蜂胶黄酮处理1 h;对照组不添加药物。各组培养24 h后,采用Western blotting法检测细胞中的NLRP3蛋白,采用ELISA法检测细胞培养液上清中的IL-1β、IL-18。结果对照组、模型组、实验1组、实验2组、实验3组细胞中NLRP3蛋白相对表达量分别为0.26±0.04、1.12±0.15、0.98±0.11、0.65±0.07、0.52±0.06,模型组与对照组相比,P均<0.05;实验2组、实验3组与模型组相比,P均<0.05。模型组培养液上清中IL-1β、IL-18水平高于对照组,实验2组、实验3组培养液上清中IL-1β、IL-18水平低于模型组(P均<0.05)。结论蜂胶黄酮可下调ox-LDL诱导的单核细胞炎症反应模型中NLRP3蛋白的表达,降低IL-1β、IL-18分泌水平,抑制NLRP3炎性小体的活性。
Objective To explore the effects of propolis flavonoids on the activation of oxidized low-density lipoprotein（ ox-LDL） induced nucleotide-binding oligomerization domain-like receptor containing protein 3（ NLRP3） inflammasome in mononuclear cells. Methods The monocytes THP1 were cultured and divided into five groups. The experimental groups1,2,3 and the model group were treated with 100 μg / m L ox-LDL to produce mononuclear inflammatory reaction models.The monocytes of the experimental groups 1,2 and 3 were treated with 5,10 and 15 μg / m L propolis flavonoids before oxLDL,and the monocytes of the control group was treated by ox-LDL in the absence of propo1 is flavonoids. After 24 h,the expression of NLRP3 was measured by Western blotting. The levels of IL-1β and IL-18 were measured by ELISA. Results The levels of NLRP3 protein in the control group,model group and the experimental groups 1,2 and 3 were 0. 26 ±0. 04,1. 12 ± 0. 15,0. 98 ± 0. 11,0. 65 ± 0. 07 and 0. 52 ± 0. 06,respectively. The expression of NLRP3 protein was significantly increased in the model group as compared with that in the control group（ all P〈0. 05）. The expression of NLRP3 protein in the experimental groups 2 and 3 was significantly decreased as compared with that of the model group（ all P〈0. 05）. The levels of IL-1β and IL-18 was significantly higher in the model group as compared with those of the control group,and the levels of IL-1β and IL-18 in the experimental groups 2 and 3 was significantly decreased as compared with those of the model group（ all P〈0. 05）. Conclusion Propolis flavonoids may down-regulate the expression of NLRP3 protein in the ox-LDL induced inflammatory response of the mononuclear cells,decrease the levels of IL-1β and IL-18 and inhibit the activation of NLRP3 inflammasome.
出处
《山东医药》
CAS
北大核心
2015年第39期10-12,共3页
Shandong Medical Journal
基金
国家自然科学基金资助项目(81202979)
关键词
蜂胶黄酮
核苷酸结合寡聚化结构域样受体3
单核细胞
炎症反应
白细胞介素1Β
白细胞介素18
propolis flavonoids
nucleotide-binding oligomerization domain-like receptor containing protein 3
mono-cytes
inflammatory response
interleukin-1β
interleukin-18
