摘要
目的 探讨肿瘤转移相关基因1（MTAl）、基质金属蛋白酶-9（MMP-9）和血管内皮生长因子C（VEGF-C）在胃腺癌中的表达及其临床病理意义.方法 采用免疫组织化学法检测120例胃腺癌中MTA1、MMP-9和VEGF-C的表达,另取30例癌旁正常胃黏膜组织作为对照.结果 胃腺癌组织中MTA1、MMP-9和VEGF-C的阳性表达率分别为76.7％、71.7％和64.2％,显著高于癌旁正常黏膜组织中的33.3％、36.7％、30.0％（P均＜0.05）,且MTA1、MMP-9和VEGF-C蛋白的表达在胃癌侵及浆膜组、淋巴结转移组和临床Ⅲ～Ⅳ期分别较未侵及浆膜组、淋巴结无转移组和Ⅰ～Ⅱ期显著增高（P均＜0.05）,MTA1蛋白表达分别与MMP-9（rs=0.352,P=0.002）和VEGF-C蛋白（rs=0.306,P=0.012）表达呈正相关.结论 MTA1、MMP-9和VEGF-C在胃腺癌组织中表达增高,提示其三种指标可能在胃腺癌的侵袭转移中发挥重要作用.MTA1可能通过调控MMP-9、VEGF-C的表达而促进肿瘤的浸润、侵袭和淋巴转移.
Objective To investigate the expression and clinicopathological significance of metastasis-associated 1 （MTA1）, matrix metalloproteinase-9 （MMP-9） and vascular endothelial growth factor-C （VEGF-C） in a gastric adenocarcinoma.Methods The expressions of MTA1, MMP-9, and VEGF-C were detected with immunohistochemical method in 120 cases of gastric adenocarcinoma tissues and 30 cases of normal gastric tissues.Results The positive percentage of MTA1, MMP-9, and VEGF-C proteins in gastric adenocarcinomas was 76.7% , 71.7% , and 64.2% , respectively, which was significantly higher than that （33.3%, 36.7%, and 30.0%） in normal gastric mucosa tissues （P 〈0.05）.The expressions of MTA1, MMP-9, and VEGF-C were significantly higher in gastric carcinoma invasion serous membrane, lymph node metastasis, clinical Ⅲ ～ than that in non-invasion serous membrane, non-lymph node metastasis, and clinical Ⅰ ～ Ⅱ （P 〈 0.05）.The expression of MTA1 had significantly positive correlation with that of MMP-9 （P 〈 0.05） and VEGF-C （P 〈 0.05） in gastric adenocarcinomas.Conclusions The expressions of MTA1, MMP-9, and VEGF-C were higher in gastric adenocarcinomas than normal gastric mucosa tissues.They may play an important role in the invasion and metastasis of gastric carcinomas.MTA1 may promote infiltration, invasion, and lymph node metastasis of gastric carcinoma through regulation of the expressions of MMP-9 and VEGF-C.The combined detection of MTA1, MMP-9, and VEGF-C expressions has important value to judge the grade malignance of gastric adenocarcinomas.
出处
《中国医师杂志》
CAS
2015年第10期1494-1497,共4页
Journal of Chinese Physician
基金
基金项目：山西省卫生计生委科研基金资助项目（2014140）
