摘要
目的:研究压榨美洲大蠊主要成分含量变化,并考察压榨美洲大蠊药材对CCl4致小鼠肝损伤保护作用,为该药材的临床应用提供参考。方法:采用压榨法去除美洲大蠊油脂,利用茚三酮显色法和苯酚-硫酸法分别测定美洲大蠊药材中总氨基酸及总多糖含量,通过CCl4致小鼠急性肝损伤模型考察压榨美洲大蠊的药效。结果:压榨美洲大蠊中油脂质量分数8.5%,总氨基酸及总多糖质量分数分别为13.334,21.06 mg·g-1,压榨美洲大蠊显著降低CCl4肝损伤小鼠的肝重系数,显著降低小鼠血清中丙氨酸转氨酶、天冬氨酸转氨酶含量。结论:美洲大蠊经炮制减毒后,炮制品具有抗肝损伤药效。
Objective： To investigate content change of main ingredients in Periplaneta americana by mechanical pressing,and investigate protective effect of pressed P. americana on liver injury in mice induced by CCl4. Method： Oil in P. americana was removed by mechanical pressing, ninhydrin colorimetry and phenolsulfuric acid method were used to measure contents of total amino acids and total polysaccharides in P. americana,respectively. Efficacy of pressed P. americana was investigated by acute liver injury model of mice induced with CCl4. Result： Content of oil in pressed P. americana was 8. 5%, contents of total amino acids and total polysaccharides were 13. 334,21. 06 mg·g- 1,respectively. Pressed P. americana can significantly reduce liver weight coefficient of mice with liver injury and significantly decrease contents of alanine aminotransferase and aspartate aminotransferase in serum of mice. Conclusion： After processing attenuated,processed product of P.americana has efficacy of anti-liver injury.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第21期20-23,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
美洲大蠊
肝损伤
总多糖
氨基酸
油脂
Periplaneta americana
liver injury
total polysaccharides
amino acids
oil
