摘要
目的探讨髓过氧化物酶（cMPO）在急性髓系白血病（AML）患者中的表达及其在诊断分型中的意义。方法采用CD45／SSC双参数散点图设门方法对502例AML患者进行八色流式细胞术免疫表型分析，观察患者白血病细胞cMPO表达的阳性率和阳性强度。结果502例AML患者cMPO总体阳性率为58．0％，其中阳性占21．5％，弱阳性占34．1％，部分阳性占2．4％；阴性占42．0％。各亚型中，AML伴t（15；17）（q22；q12）／PML．RARa的cMPO阳性率最高，为100％，阳性强度多数接近正常粒细胞水平；其次为AML伴t（8；21）（q22；q22）／RUNXl-RUNX1T1，阳性率为91．4％，阳性强度多为弱阳性；AML微分化型和急性巨核细胞白血病患者cMPO表达皆为阴性；余各亚型阳性率在22．7％～76．2％。结论各亚型AMLcMPO的阳性率及阳性强度存在显著差异。
[Abstract] Objective To investigate the myeloperoxidase （cMPO） expression pattern by flow cytometry （FCM） in patients with acute myeloid leukemia （AML） and its role in classifying AML. Methods Eight-color multiparametric FCM with CD45/SSC gating was used to determine the cMPO expression in 502 AML patients. Results The positive rate of cMPO in all patients was 58.0%, in which the proportion of normal positivity, dim positivity and partial positivity was 21.5%, 34.1% and 2.4%, respectively. The remaining cases （42.0%） were all negative. In AML with t （15;17）（q22;q12）/PML- RARer, the positive rate was the highest （100%） and the intensity was similar to that of the normal granular leukocytes, followed by AML with t（8;21 ） （q22;q22）/RUNX1-RUNX1T1, the positive rate was 91.4% and the intensity was mostly dim. AML with minimal differentiation and acute megakaryoblastic leukemia were all cMPO negative. The positive rates of cMPO in the remaining subtypes were between 22.7% and 76.2%. Conclusion The positive rate and intensity of cMPO were significantly different among differentsubtypes of AML.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2015年第11期906-911,共6页
Chinese Journal of Hematology
