摘要
目的观察偏钒酸钠亚慢性经口染毒所致大鼠学习记忆损伤情况,探讨其对纹状体神经细胞凋亡的影响。方法无特定病原体级健康雄性SD大鼠32只,随机分为对照组及低、中、高剂量组,每组8只。分别予质量浓度为0.0、0.5、1.0、2.0 g/L偏钒酸钠水溶液经口饮水染毒3个月后,采用Morris水迷宫实验测试大鼠学习记忆能力,分离大鼠纹状体观察神经细胞形态学改变并以流式细胞仪检测其神经细胞凋亡率。结果对照组和低、中、高剂量组大鼠平均逃避潜伏期分别为(37.43±8.82)、(45.71±12.44)、(59.74±22.91)、(68.45±12.06)s;中剂量组大鼠逃避潜伏期高于对照组(P<0.05),高剂量组大鼠逃避潜伏期分别高于对照组和低剂量组(P<0.05)。4组大鼠穿越平台次数中位数(M)分别为3.5、3.0、2.0和2.0次;中和高剂量组穿越平台次数均低于对照组(P<0.05)。4组大鼠脑纹状体神经细胞早期凋亡率M分别为2.15%、5.45%、9.50%和13.55%,晚期凋亡率M分别为0.05%、0.15%、0.35%和0.75%,总凋亡率M分别为2.35%、5.60%、9.80%和14.65%;中和高剂量组纹状体神经细胞早期凋亡率、总凋亡率均分别高于对照组(P<0.05)。病理组织学检查结果显示:大鼠纹状体组织疏松、水肿、空泡变性,可见细胞凋亡形态。Spearman相关分析显示:钒染毒大鼠的逃避潜伏期与纹状体神经细胞早期凋亡率和总凋亡率均呈正相关[Spearman相关系数(rS)分别为0.615和0.565,P<0.05];穿越平台次数与纹状体神经细胞早期凋亡率和总凋亡率均呈负相关(rS分别为-0.450和-0.483,P<0.05)。结论偏钒酸钠可通过介导纹状体神经细胞凋亡增加而损伤大鼠学习记忆能力。
Objective To observe the damage of learning and memory ability in rats induced by subchronic oral exposure to sodium metavanadate and to investigate its effect on the apoptosis in the striatum nerve cells. Methods Thirty-two specific pathogen free healthy male SD rats were randomly divided into 4 groups： control group,low-,medium-,and highdose groups. Each group had 8 rats and were given 0. 0,0. 5,1. 0 and 2. 0 g / L sodium metaranadate respectively in drinking water. Three months later, the learning and memory ability was measured by Morris water maze test.Morphological changes of neurons in the striatum were observed and the apoptosis rate was detected by flow cytometry.Results The escape latency of control,low-,medium-,high-dose groups were（ 37. 43 ± 8. 82）,（ 45. 71 ± 12. 44）,（ 59. 74 ± 22. 91） and（ 68. 45 ± 12. 06） s respectively. The escape latency of medium-dose group was higher than that of the control group（ P〈0. 05）; the escape latency of high-dose group was higher than those of control group and low-dose group respectively（ P〈0. 05）. The frequency median（ M） of crossing platform of control,low-,medium- and high-dose groups were 3. 5,3. 0,2. 0 and 2. 0 times,respectively. The frequency of crossing platform of medium- and high-dose groups were lower than that of the control group（ P〈0. 05）. The early M apoptosis rates of striatum nerve cells in the 4groups were 2. 15%,5. 45%,9. 50% and 13. 55%,respectively,the late M of apoptosis rates were 0. 05%,0. 15%,0. 35% and 0. 75%, respectively, and the total M apoptosis rates were 2. 35%,5. 60%,9. 80% and 14. 65%,respectively. The early apoptosis rate and total apoptosis rate of medium- and high-dose groups were higher than those of the control group（ P〈0. 05）. Histopathologic examination showed the loose,edema and vacuoles degeneration striatum tissue,with visible apoptotic morphology. Spearman correlation analysis showed that escape latency was positively related with the early and total striatum apoptosis rates [Spearman correlation coefficient（ rS） were 0. 615 and 0. 565,P〈0. 05],and the frequency of crossing platform was negatively correlated with the early and total striatum apoptosis rate（ rSwere- 0. 450 and- 0. 483,P〈0. 05）. Conclusion Sodium metavanadate can damage the learning and memory ability of rats by increasing the nerve cell apoptosis in rat striatum.
出处
《中国职业医学》
CAS
北大核心
2015年第5期516-520,共5页
China Occupational Medicine
基金
贵州省联合基金(CK-1002)
