摘要
目的:研究中药肾糖组方对糖尿病肾病大鼠C-Ⅳ、FN表达影响,探讨其在DN大鼠肾保护中的作用机制。方法:采用高脂饲料喂养+STZ腹腔注射制作DN大鼠模型。分正常对照组、模型对照组、中药治疗组、西药治疗组(洛汀新组)、雷公藤治疗组、中西药治疗组(肾糖组方+洛汀新组),测定大鼠24 h尿蛋白定量,免疫组化方法测定大鼠肾组织中C-Ⅳ、FN表达。结果:经中药肾糖组方治疗2个月后,24 h尿蛋白定量各治疗组均明显低于模型组(P<0.05);大鼠模型组C-Ⅳ、FN分别呈现高阳性表达,明显高于各治疗组(P<0.05)。结论:中药肾糖组方对DN大鼠有一定的治疗效果,可以改善肾功能,延缓糖尿病肾脏损伤,其可能机制是抑制了大鼠肾纤维化进展,与抑制了C-Ⅳ、FN在大鼠肾组织中表达有关。
Objective： To investigate the effect of Traditional Chinese medicine kidney sugar formula on expression of Fibronectin（ FN） and typeⅣ collagen（ C- Ⅳ） of renal tissue in STZ- induced diabetic nephropathy rats. Methods： Fed with high fat diet ＋ STZ produced by intraperitoneal injection in rats with diabetic nephropathy. Rats were randomly divided into five groups： normal control group,model control group,Chinese medicine group（ Traditional Chinese medicine kidney sugar formula）,western medicine group（ Lotensin group） and Chinese and Western medicine group（ Traditional Chinese medicine kidney sugar formula ＋ Lotensin group）. Rats were determined 24 h urinary protein and were measured by immunohistochemistry in rats C- Ⅳ,FN expression. Results： After treatment of two months with Traditional Chinese medicine kidney sugar formula,24 h urinary protein in the treatment groups were significantly lower than the model group（ P〈0. 05）; and C- IV and FN of Renal Tissue in the model rats showed high expression respectively,significantly higher than every treatment groups（ P〈0. 05）. Conclusion： Renal sugar granule has a certain therapeutic effect on STZ- induced Diabetic Nephropathy Rats. Renal sugar granule can improve kidney function and delay diabetic kidney damage. The possible mechanism is the inhibition of the progress of renal fibrosis in rats,which was connected with the inhibited expression of C- Ⅳ and FN in renal tissue in rats.
出处
《中国中西医结合肾病杂志》
2015年第10期858-860,I0001,共4页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
浙江省中医药管理局基金资助项目(No.2010ZB155)
关键词
中药肾糖组方
DN大鼠
C-Ⅳ
FN
Traditional Chinese medicine kidney sugar formula Diabetic nephropathy in rats C-Ⅳ FN
