摘要
目的对细叶益母草Leonurus sibiricus的化学成分和血小板聚集活性进行研究。方法细叶益母草提取液采用大孔树脂、MCI、反相C18、硅胶、Sephadex LH-20柱色谱及重结晶等方法进行分离纯化,所得化合物的结构通过波谱分析和TLC来鉴定,然后对其进行抗血小板聚集活性筛选。结果从中分离鉴定出9个化合物,分别为益母草碱（1）、丁香脂素（2）、梣皮树脂醇（3）、蒙花苷（4）、芹菜素-7-O-β-D-吡喃葡萄糖苷（5）、丁香酸乙酯（6）、对羟基苯甲酸乙酯（7）、对羟基苄乙醚（8）、β-胡萝卜苷（9）。结论化合物2~4、6、8均为首次从该植物中分离得到,其中3和6为首次从益母草属植物中分离得到。而且,化合物1、4和5对ADP诱导的血小板聚集均有明显的抑制作用。
AIM To study the chemical constituents and their anti-platelet aggregation activities of Leonurus sibiricus L. METHODS The analysis of L. sibiricus extract was carried out on macroporous resin,MCI,ODS C18,silica,Sephadex LH-20 gel column and recrystallization. The structures of isolated compounds were identified by spectral analysis and TLC. Then their anti-platelet aggregation activities were screened. RESULTS Nine compounds were isolated from this plant and identified as leonurine（ 1）,syringaresinol（ 2）,medioresinol（ 3）,linarin（ 4）,apigenin-7-O-β-D-glucopyranoside（ 5）,syringic acid ethyl ester（ 6）,ethyl 4-hydroxybenzoate（ 7）,p-hydroxybenzyl ethyl ether（ 8）,β-daucosterol（ 9）. CONCLUSION Compounds 2- 4,6 and 8 are obtained from L. sibiricus for the first time. Among them,compounds 3 and 6 are firstly isolated from genus Leonurus. In addition,compounds 1,4 and 5 can significantly inhibit platelet aggregation induced by ADP.
出处
《中成药》
CAS
CSCD
北大核心
2015年第11期2439-2442,共4页
Chinese Traditional Patent Medicine
基金
国家自然科学基金资助项目(81303209)
中国博士后科学基金资助项目(2014M562291)
四川省应用基础研究项目(2012JYZ005)
四川省科技支撑计划(2011SZ0056)
关键词
细叶益母草
化学成分
抗血小板聚集
二磷酸腺苷(ADP)
分离鉴定
Leonurus sibiricus
chemical constituents
anti-platelet aggregation
adenosine diphosphate（ADP）
isolation and identification
