摘要
目的探讨不同质量浓度臭氧水对牙周炎大鼠牙周组织中白细胞介素-6(IL-6)的表达影响。方法本研究于2013年4—8月在佳木斯大学动物实验中心进行。选用70只Wistar雄性大鼠,随机抽取5只作为正常对照组(颈椎脱臼法处死),其余大鼠均建立牙周炎模型。牙周炎模型建立后,随机抽取5只作为牙周炎未治疗组(颈椎脱臼法处死),其余牙周炎大鼠随机分为臭氧水治疗组和对照治疗组进行牙周袋冲洗。运用一般组织学方法观察牙周组织形态变化,并采用免疫组化法检测牙周组织中IL-6含量的变化。结果牙周炎未治疗组的牙周组织中IL-6表达明显高于正常对照组(P<0.05);各治疗组的牙周组织中IL-6表达均不同程度低于牙周炎未治疗组(P<0.05);在各牙周袋冲洗时间段,15 mg/L臭氧水治疗组的牙周组织中IL-6表达均低于5、10 mg/L臭氧水治疗组(P<0.05),但与对照治疗组的差异无统计学意义(P>0.05)。结论臭氧水能降低炎性牙周组织中IL-6的表达,其中质量浓度为15mg/L的臭氧水对其影响更加明显,对牙周炎有一定治疗效果。
Objective To study the effect of different concentrations of ozone water on the expression of IL-6 in periodontal tissues of rat with periodontitis. Methods A total of 70 male Wistar rats weighing（200 ± 20）g were chosen;randomly select five as normal control group （executed by cervical dislocation method） , and the rest of the rats were made model of periodontitis. When the model was established, five periodontitis rats were randomly selected as no-treatment group （executed by cervical dislocation method）. The rest of periodontitis rats were randomly divided into ozone water treatment group and control treatment group for periodontal pocket rinsing. The general conditions and pathological changes of the periodontal tissues were observed. Immunohistochemieal staining was conducted to determine the expres- sion of IL-6 in the periodontal tissues. Results The level of IL-6 in periodontal tissues of rats of no-treatment group was much higher than that in the normal control group （P 〈 0.05 ）. The level of IL-6 in the treatment groups was much lower than that in the no-treatment group （P 〈 0.05）. In each period of rinsing, at 15mg/L of ozone water treatment group, the expression of IL-6 in periodontal tissue was lower than that in other concentration groups （5mg/L, 10mg/L） （P 〈 0.05）, but there was no significant difference compared with the control treatment group. Conclusion The ozone water can reduce the IL-6 level in periodontal tissues in rats and the effect is more obvious in concentration of 15mg/L, which has certain treatment effect on periodontitis.
出处
《中国实用口腔科杂志》
CAS
2015年第10期608-611,共4页
Chinese Journal of Practical Stomatology
