摘要
目的探讨姜黄素对DSS诱导溃疡性结肠炎小鼠T淋巴细胞亚群及BTLA表达的影响。方法采用葡聚糖硫酸钠法(DSS)制备UC小鼠模型,小鼠随机分为4组:对照组、DSS模型组、地塞米松组、姜黄素组,通过腹腔注射药物作用,应用流式细胞仪检测T淋巴细胞亚群和细胞因子表达的百分率。结果姜黄素组与DSS模型组比较,小鼠外周血CD4+细胞数明显升高,CD8+细胞数明显降低,CD4+/CD8+比值明显升高(Р<0.01);小鼠脾脏Th1+细胞、Tc1+细胞比例和Th1+/Th2+比值、Tc1+/Tc2+比值明显降低,而Th2+细胞明显升高(P<0.05);小鼠外周血、脾脏CD4+T细胞上BLTA+的表达明显增高(P<0.01)。结论姜黄素对DSS诱导UC小鼠CD4+T细胞亚群及BTLA表达显著升高,可能是通过调节CD4+/CD8+T淋巴细胞亚群间的平衡,抑制Th1型/Tc1型细胞分化,促使CD4 T淋巴细胞表面BTLA表达上调,降低免疫反应,发挥抗炎及治疗UC作用。
Objective To investigate the effect of curcumin on T lymphocyte subsets and BTLA expression in mice with ulcerative colitis induced by DSS. Methods 5% Dextran sulfate sodium（ DSS） was used for the establishment of ulcerative colitis（ UC） rat model,and then UC rat model was divided into four groups： control group,DSS model group,dexamethasone group and curcumin group（ 30 mg / kg）. The proportion of T lymphocyte subsets in peripheral blood and expression of cytokines were calculated by flow cytometry.Results In this study,we observed an increase in CD4 ＋ T cell was associated with treatment of curcumin（ P〈0. 01）. Contrary to CD4 ＋ T cell,the number of CD8- T cell and ratio of CD4 ＋ / CD8 ＋ in curcumin group was lower than model group（ P〈0. 01）. The loss of Th1 ＋,Tc1 ＋,Th1 ＋ / Th2 ＋ and Tc1 ＋ / Tc2 ＋ was also observed in curcumin group（ P〈0. 05）. The high level of BTLA expression was showed in curcumin group（ P〈0. 01）. Conclusions Curcumin shows obvious effect on CD4 ＋ T cell subsets and BTLA expression,and the therapeutic mechanism might be related with the balance of CD4 ＋ / CD8 ＋ T cell,the inhibition of Th1 / Tc1 differentiation and up- regulation of BTLA expression.
出处
《齐齐哈尔医学院学报》
2015年第29期4375-4378,共4页
Journal of Qiqihar Medical University
基金
广东省科技计划项目(2011B061300098)
湛江市科技计划项目(2014B01011)
