摘要
目的观察地塞米松对脂多糖大鼠早期急性肝损伤时肝组织髓样分化蛋白2(MD-2)基因及核因子-κB(NF-κB)蛋白表达的影响。方法 15只雄性SD大鼠被随机分为正常组、脂多糖组和治疗组(脂多糖+地塞米松组),每组各5只。自大鼠尾静脉穿刺置留置针以备给药,正常组自大鼠尾静脉注射生理盐水10 ml;脂多糖组静脉注射脂多糖10 mg/kg(10 min以上注射完);治疗组静脉注射脂多糖10 mg/kg(10 min以上注射完)和地塞米松0.1 mg/kg。所有大鼠均于注射后1 h被处死,抽取左心室血行生化检测谷丙转氨酶(ALT)、谷草转氨酶(AST)。剪取部分肝右叶制成肝组织匀浆液(保存于-20℃条件下以备用),以检测肝组织MD-2基因,另取部分肝组织检测NF-κB蛋白的表达水平。结果 (1)正常组大鼠转氨酶值波动于正常范围,脂多糖组大鼠ALT、AST明显增高,与脂多糖组比较,治疗组转氨酶值明显下降,差异有统计学意义(P<0.05);(2)正常的肝组织中可见少量MD-2基因、NF-κB蛋白表达;与正常组比较,脂多糖组和治疗组肝组织MD-2基因及NF-κB蛋白的表达水平均明显,差异均有统计学意义(P<0.05),但治疗组MD-2基因及NF-κB蛋白的表达较脂多糖组明显减少,差异均有统计学意义(P<0.05)。结论早期脂多糖大鼠发生急性肝损伤时,地塞米松对其有明显的治疗作用,其治疗机制可能与MD-2基因和NF-κB蛋白表达水平的减少有关。
Objective To observe the effect of dexamethasone on expression of myeloid differentiation pro-tein-2 （MD-2） genes and nuclear factor-κB （NF-κB） in liver tissue at the early stage of lipopolysaccharide （LPS）-in-duced rats. Methods Fifteen male SD rats were randomly divided into normal group, LPS group and treatment group （LPS＋dexamethasone group）, with 5 rats in each group. The rats were punctured catheter from tail vein to pre-pare for the administration. Normal group was injected with 10 ml saline, LPS group was injected with LPS （10 mg/kg） for more than 10 min, and the treatment group was injected with LPS （10 mg/kg, for more than 10 min） and dexameth-asone （0.1 mg/kg）. The rats were killed at 1 h after injection. The blood from the left ventricle was tested by biochemi-cal detection for alanine aminotransferase （ALT） and aspartate aminotransferase （AST）. A part of the right lobe of liver tissue were extracted to store at-20℃in order to detect the expressions of MD-2 gene, and another part of the liver tissue were extracted to detect the level of NF-κB protein. Results （1） ALT and AST levels of the normal group were within the normal range, and the ALT and AST levels in LPS group were significantly increased. Compared with LPS group, the levels in treatment group were significantly decreased, with statistically significant difference between the two groups （P〈0.05）. （2） The expressions of MD-2 gene and NF-κB protein were revealed low in the normal group, while the expressions in LPS group and treatment group were relatively high, showing statistically significant difference with the normal group （P〈0.05）. The expressions in the treatment group were significantly decreased com-pared with LPS group （P〈0.05）. Conclusion Dexamethasone has significant clinical effects on acute liver injury at the early stage of LPS-induced rats. The mechanism may be associated with decreased expression level of MD-2 gene and NF-κB protein.
出处
《海南医学》
CAS
2015年第21期3121-3123,共3页
Hainan Medical Journal
基金
四川省教育厅自然科学重点项目[川教函(2008)760号:09027]
泸州医学院青年基金资助项目(编号:泸医院科[2009]1号-09008)
