摘要
目的分析100例大肠癌患者VEGF、EGFR、Ki-67及P53基因表达与肿瘤浸润转移的相关性。方法选取100例行肠电子内镜活检组织,病理确诊为大肠癌行手术治疗的患者为研究对象,根据患者大肠癌Dukes分期分成两组,其中A组为Dukes分期D期57例,B组为Dukes分期A+B+C期43例,随机从B组中选取15例,取手术标本切缘正常大肠黏膜做对照,为C组,免疫组化Envision法对组织中VEGF、EGFR、Ki-67、P53进行检测。观察三组标本相关指标表达差异。结果 A组VEGF、EGFR、Ki-67、P53表达总阳性率为82.5%、80.7%、84.2%、82.5%,B组表达总阳性率为76.7%、72.1%、69.8%、67.4%,C组组表达总阳性率为13.3%、13.3%、6.7%、6.7%,三组VEGF、EGFR、Ki-67、P53表达率等级资料分析差异有统计学意义(P<0.05)。结论 VEGF、EGFR、Ki-67及P53基因表达与大肠癌的浸润转移有着密切的关系,检测VEGF、EGFR、Ki-67及P53的表达情况对判断大肠癌预后及指导治疗有一定的参考意义。
Objective To observe and analyze the expression of VEGF,EGFR,Ki- 67 and P53 in correlation withcolorectal cancer invasion and metastasis in 100 cases of patients with colorectal cancer. Methods A total of 100 routineintestinal endoscopy biopsy patients,pathological diagnosis as colorectal cancer,who underwent surgery wrer selected as thestudy subjects,according to Dukes stages divided into two groups,57 cases of Dukes stage D as group A,43 cases of Dukesstage A＋B＋C as group B,and 15 cases randomly selected for normal colorectal mucosa obtained from the surgical specimens ascontrast,as group C,and the expression of VEGF,EGFR,Ki-67,P53 in the tissues were detected by immunohistochemicalEnvision method. The expression differences in three groups were observed. Results The total positive rates of VEGF,EGFR,Ki-67,P53 in group A were 82.5%,80.7%,84.2%,82.5%,the total positive rates of VEGF,EGFR,Ki-67,P53 ingroup B were 76.7%,72.1%,69.8%,67.4%,the total positive rates of VEGF,EGFR,Ki-67,P53 in group C were 13.3%,13.3%,6.7%,6.7%,the expression rates of VEGF,EGFR,Ki-67,P53 had significant differences among the three groups（P0.05）. Conclusion The expression of VEGF,EGFR,Ki-67 and P53 has a close correlation with colorectal cancer invasionand metastasis,measuring the expression of VEGF,EGFR,Ki- 67 and P53 is helpful in the treatment and prognosis incolorectal cancer.
出处
《中国热带医学》
CAS
2015年第11期1369-1371,共3页
China Tropical Medicine
基金
广东省佛山市卫生和计生局医学科研项目(No.2015011)
