摘要
目的:探讨艾灸胃经穴防治慢性萎缩性胃炎(chronic atrophic gastritis,CAG)癌前病变的分子机制。方法:50只雄性SD大鼠随机分为5组,分别为正常组、模型组、胃经穴组、对照点组、维酶素组,每组10只。除正常组外,余组复制CAG癌前病变大鼠模型。正常组和模型组大鼠每天捆绑30min;胃经穴组和对照点组大鼠每天捆绑,分别艾灸胃经穴("梁门""足三里")或对照点30min;维酶素组每天灌胃维酶素,均每天1次,共20周。光镜下观察各组大鼠胃黏膜病理形态学变化,酶联免疫吸附法(enzyme linked immuno sorbent assay,ELISA)检测胃黏膜细胞表皮生长因子(epidermal growth factor,EGF)、转化生长因子-α(transforming growth factor alpha,TGF-α)、血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)、胃黏膜增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)蛋白、核仁组成区嗜银蛋白(argyrophilic protein of nucleolar organizer reigons,Ag-NORs)的表达。结果:与正常组比较,模型组大鼠胃黏膜细胞出现异型增生,胃黏膜细胞EGF、TGF-α、PCNA、VEGF、Ag-NORs表达显著升高(均P<0.05);与模型组比较,胃经穴组、对照点组、维酶素组大鼠胃黏膜渐趋恢复,胃黏膜细胞EGF、TGF-α、PCNA、VEGF、Ag-NORs表达渐趋下降,其中胃经穴组大鼠胃黏膜修复和胃黏膜细胞增殖因子表达下降尤为显著(均P<0.05)。结论:艾灸胃经穴能明显降低CAG癌前病变大鼠胃黏膜细胞增殖因子的表达,抑制胃黏膜细胞的异型增生,促进胃黏膜的修复。
Objective To explore the molecular mechanism of moxibustion at stomach meridian acupoints for precancerous lesions of chronic atrophic gastritis （CAG）. Methods Fifty male SD rats were randomly divided into a normal group, a model group, a stomach meridian group, a control point group and a vitaeoenzyme group, 10 rats in each group. The CAG precancerous lesion model was made in all the groups except the normal group. The rats in the normal group and model group were bundled for 30 min per day; the rats in the stomach meridian group and control point group were bundled and treated with moxibustion at stomach meridian acupoints or control points for 30 rain per day; the rats in the vitacoenzyme group were treated with intragastric administration of vitacoenzyme, once per day. All the treatment was given for 20 weeks. The pathological morphological change of gastric mueosa was observed under optical microscope; the expression of epidermal growth factor （EGF）, transforming growth factor alpha （TGF-α）, vascular endothelial growth factor （VEGF）, gastric mucosal proliferatig cell nuclear antigen （PCNA）, argyrophilie protein of nucleolar organizer regions （Ag-NORs） in gastric mucosal ceils were detected by enzyme linked immuno sorbent assay （ELISA）. Results Compared with the normal group, in the model group the gastric mucosal cells showed dysplasia and the expression of EGF, TGF-α, PCNA, VEGF, Ag-NORs in gastric mucosa cells in the model group was increased significantly （all P〈0.05）. Compared with the model group,the gastric mucosa lesion gradually recovered and the expression of EGF, TGF-α, PCNA, VEGF, Ag-NORs in gastric mucosal cells was gradually decreased in the stomach meridian group, control point group and vitacoenzyme group, in which the stomach meridian group had the most significant effects （all P〈0.05）. Conclusion Moxibustion at stomach meridian acupoints can obviously decrease the expression of cell proliferative factors in gastric mucosa in rats with CAG precancerous lesions, inhibit the gastric mucosal cell dysplasia, and promote the recovery of gastric mucosa.
出处
《中国针灸》
CAS
CSCD
北大核心
2015年第12期1269-1273,共5页
Chinese Acupuncture & Moxibustion
基金
国家自然科学基金项目:81260556
81473751
关键词
慢性萎缩性胃炎
癌前病变
艾灸
胃经穴
细胞增殖
chronic atrophic gastritis （CAG）
precancerous lesion
moxibustion
stomach meridian acupoint
cell proliferation
