摘要
【目的】明确梨腐烂病菌强、弱致病力菌株在梨树叶片组织中的侵染及扩展情况。【方法】利用农杆菌介导的遗传转化(agrobacterium-mediated transformation technique,ATMT)方法对梨腐烂病菌强、弱致病力菌株进行绿色荧光蛋白(green looresent protein,GFP)标记,并筛选出和野生型菌株比较在生长速度、培养特性以及致病力都没有发生显著变化的阳性转化子菌株;利用荧光显微技术观察其在梨树叶片组织中的侵染和扩展,比较强、弱致病力菌株的侵染差异。【结果】强、弱致病力菌株在叶片上侵染存在差异。菌丝主要在叶片的上表皮扩展,菌丝扩展前端的叶片组织颜色发生变化,形成一段变色带,强致病力菌株侵染形成的变色带较弱致病力菌株形成的变色带宽,强致病力菌株菌丝在叶片组织上的分布较稀疏。【结论】梨腐烂病菌菌丝主要在叶片的上表皮组织扩展;强致病力菌株的侵入能力较强,其在叶片上扩展时菌丝分布较稀疏,扩展前端形成的变色带宽。
[Objective]The processes of infection and expansion of strong and weak virulent strains of Valsa pyri in pear leaf tissues were carried out in this study. [Methods] Using the agrobacterium-mediated transformation technique （ATMT）, strong and weak virulent strains were marked with GFP. Via comparing with their parent strains in growth rate and biological characteristics, the no significantly changed positive transformations were screened out for observing the processes of expansion in different pear tissues and finding out the difference in infection between the transformations of strong and weak virulent strains through fluorescence microscopy. [ Results ] There were differences between the transformations of strong and weak virulent strains in the infection and expansion in pear leaves. On the infected leaves, fungal hyphae expanded on the surface of upper epidermis. The color of the leaf tissue in the front of the growth of the mycelium was changed, and a discolored band was formed. The belts caused by transformations of strong virulent strains were wider than those by transformations of weak virulent strains. [ Conclusion ] Hyphae of pear V. pyri expanded on the upper epidermis of leaf, and the expansive ability of virulent strains was stronger than that of weak virulent ones.
出处
《果树学报》
CAS
CSCD
北大核心
2015年第6期1195-1200,I0004,I0005,共8页
Journal of Fruit Science
基金
国家现代农业产业技术体系建设专项资金项目(CARS-29-10)
国家公益性行业(农业)科研专项项目(201203034-02
200903004-05)
关键词
梨腐烂病菌
农杆菌介导遗传转化
绿色荧光蛋白标记
侵染
扩展
Pear Valsa canker
Agrobacterium-mediated transformation technique （ATMT）
Green looresent protein（GFP）tagged
Infection
Expansion
