摘要
目的:观察不同电针刺激量对雷公藤甲素致大鼠急性肝损伤的影响,并初步探讨其机制。方法:将50只SD大鼠随机分为空白组、模型组、电针20 min组、电针30 min组及电针40 min组,以雷公藤甲素制备急性肝损伤模型。电针组电针"足三里"穴,每日1次,连续5 d;检测血清ALT、AST水平及肝匀浆组织MDA、(白介素)IL-10、(肿瘤坏死因子)TNF-α、(硫化氢)H2S水平,观察肝脏组织形态学变化。结果:模型组大鼠血清ALT、AST及肝组织MDA、TNF-α明显高于正常组(P<0.01,P<0.05),IL-10、H2S含量降低(P<0.01,P<0.05);电针组血清ALT水平及肝匀浆组织MDA、TNF-α水平较模型组均有降低(P<0.05,P<0.01),以电针30 min最低;IL-10较模型组升高(P<0.05,P<0.01),但组间差异无统计学意义(P>0.05),H2S较模型组升高(P<0.05,P<0.01)。结论:电针组均能减轻雷公藤甲素对肝组织的损伤,以电针30 min组作用较为明显,其机制可能与调节炎症反应、抑制脂质过氧化有关。
Objective：To observe the effect of electro-acupuncture with different stimulation Intensities on acute hepatic injury caused by triptolide in rats and to explore the mechanism. Methods：A total of 50 SD rats were equally randomized into normal group,model,（electro-acupuncture）EA 20 minutes,EA 30 minutes and EA 40 minutes;the acute hepatic injury model was established by giving the rats triptolide. Electro-acupuncture was applied to＂Tsusanli＂（ST 36）,once daily for 5 days beginning from the first day of modeling. Detect the level of ATL,AST,MDA,H2 S,（interleukin）IL-10 and（Tumor Necrosis Factor α）TNF-αcontents,and to observe the pathological changes of hepatocytes. Results：In comparison with the normal group,ATL,AST,MDA and TNF-αcontents were significantly increased and IL-10 contents were decreased in the model group（P〈0.01,P〈0.05）.Compared to the model group,ATL,AST,MDA and TNF-αcontents were considerably downregulated,and it is the most significant in EA 30 min group;IL-10 content was obviously up-reulated in EA groups（P〈0.05,P〈0.01）,but there were no remarkable difference among the three groups,H2 S content was also obviously up-reulated in EA groups（P〈0.05,P〈0.01）. Conclusion：Electroacupuncture can relieve liver injury in acute hepatic injury caused by triptolide rats,and it is the most significant in EA 30 min group. The mechanism may possibly relate to the regulation of inflammatory response and inhibition of lipid peroxidation.
出处
《辽宁中医药大学学报》
CAS
2015年第12期23-25,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
江苏省中医药局资助项目(LB13049)
关键词
电针
不同刺激量
肝损伤
雷公藤甲素
抗炎因子
促炎因子
electro-acupuncture
different stimulation intensities
hepatic injury
triptolide
anti-inflammtory cytokine
serum inflammatory cytokines
