摘要
目的：观察芝莲化癥不同组方对移植肝癌小鼠瘤体质量、抑瘤率、巨噬细胞吞噬率、吞噬指数、半数溶血值、淋巴细胞转化率水平的影响。方法：选择雄性8~10周龄健康清洁级C57小鼠50只,采用随机数字表法分为模型组、芝莲化癥药效组分组、芝莲化癥配方颗粒组、芝莲化癥膏方组、健康对照组各10只。除健康组外,各组小鼠腋下皮下接种肝癌Hepa 1-6瘤株。接种1周后给予芝莲化癥灌胃,连续治疗10 d。观察各项指标水平。结果：中药治疗各组较模型组瘤体质量明显下降,其中芝莲化癥配方颗粒组、芝莲化癥膏方组与模型组比较,差异有统计学意义（P〈0.05）。芝莲化癥膏方组抑瘤率（45.22±2.70）%较芝莲化癥药效组分组（35.22±2.46）%、芝莲化癥配方颗粒组（42.68±2.13）%升高,但组间比较,差异无统计学意义（P〉0.05）。中药治疗各组吞噬率、吞噬指数（0.60±0.03、0.63±0.04）较模型组明显提高,中药治疗各组与模型组（0.51±0.01）吞噬指数比较,差异有统计学意义（P〈0.05）。中药治疗各组半数溶血值较模型组均有升高,芝莲化癥膏方组与模型组（19.68±1.93 vs15.20±2.40）比较,差异有统计学意义（P〈0.05）。中药治疗各组淋巴细胞转化率均有升高,芝莲化癥配方颗粒组（40.61±3.50）%、芝莲化癥膏方组（41.22±3.61）%与模型组（25.06±1.60）%比较,差异有统计学意义（P〉0.05）。结论：芝莲化癥方药不同组方能调节巨噬细胞吞噬率、淋巴细胞转化率,抑瘤效果明显,尤其是芝莲化癥膏方具有显著的调节免疫功能抗肿瘤功效。
Objective ：To investigate effect of Zhilianhuazheng different prescriptions on tumor weight and inhibition rate, macropbage phagocytosis rate, phagocytic index, serum hemolysin, lymphocyte transformation rate of transplanted liver cancer mice. Methods： Choosing male 8 - 10 weeks old, clean grade healthy C57 mice 50, were randomly divided into the model group, Chinese medicine group 1 （Zhilianhuazheng efficacy components group）, Chinese medicine group 2 （Zhilianhuazheng formula granules group）, Chinese medicine group 3 （Zhilianhuazheng cream formula group） and healthy control group with 10 rats in each group. In addition to the healthy group, the axillary subcutaneous inoculation of 1 - 6 Hepa tumor. After a week of inoculation to the stomach, 10 days of con- tinuous treatment, observed the indicators level. Results： The weight of the Chinese medicine treatment group was significantly de- creased compared with the model group, and comparison of Chinese medicine 2, 3 groups and the model group showed that the differ- ence was significant （ P 〈 0.05 ）. Anti-tumor rate of Chinese medicine group 3 was （45.22 ± 2.70 ） % , Chinese medicine group 1 （ 35. 22 ± 2.46 ） % , Chinese medicine group 2 （42.68 ± 2.13 ） %, but there was no significant difference between the groups （ P 〉 0. 05 ）. The phagocytic rate and phagocytic index in the Chinese medicine group were （0.60 ± 0.03, 0.63± 0.04） which were significantlyimproved compared with the model group, and there was significantly different between Chinese medicine each group and the model group in the phagocytic index （ P 〈 0.05 ）. Serum hemolysin in Chinese medicine groups were significantly higher compared with the model group, and the difference between Chinese medicine group 3 （ 19.68 ±1.93 ） and the model group （ 15.20± 2.40） was statisti- cally significant（ P 〈 0.05 ）. The lymphocyte transformation rate of the Chinese medicine groups were increased, Chinese medicine group 2 （40.61 ± 3.50） % and group 3 （41.22 ± 3.61 ） % , compared with the model group （25.06 ± 1.60） % had significant differ- ence（P 〈0.05）. Conclusion： Zhilianhuazheng different prescriptions can adjust the macrophage phagocytic rate and the lymphocyte transformation rate, and the tumor inhibition rate is good. Zhilianhuazheng cream formula has significant regulatory immune function of anti-tumor effect.
出处
《中医学报》
CAS
2015年第12期1703-1705,共3页
Acta Chinese Medicine
基金
河北省中医药管理局科学研究计划项目(2014091)
关键词
芝莲化癥配方颗粒
芝莲化癥膏方
益气化癥法
肝癌
小鼠
zhilianhuazheng formula granules
zhilianhuazheng cream formula
benefiting Qi and eliminating mass
liver cancer
mice
