摘要
目的：研究陕西省商洛地区妇女人乳头瘤病毒（human papillomavirus,HPV）的感染状况和亚型分布,为HPV分子流行病学和宫颈癌的防治提供依据。方法：选择2012年6月至2014年7月在商洛中心医院体检、门诊就诊的937例患者的宫颈脱落细胞标本,采用聚合酶链反应（PCR）体外扩增和脱氧核糖核酸（DNA）反向点杂交相结合的DNA芯片技术进行HPV基因分型检测。结果：HPV检出率937例标本中HPV感染566例,总感染率为60.41%（566/937）,其中高危型感染病例总检出率为51.44%（482/937）;低危型感染总检出率26.47%（248/937）;感染年龄分布以〉61岁年龄段感染率最高,为88.89%,其次是≤20岁年龄段（67.86%）,51~60岁年龄段（62.90%）,41~50岁年龄段（60.29%）,各个年龄组的感染率没有统计学差异（P〉0.05）。23种HPV基因亚型中,高危感染中以16型最高,占26.58%（277/1042）,其次是58型,占8.93%（93/1024）,66型,占6.53%（68/1024）,低危感染中以6型最高,占12.38%（129/1024）,其次是43型,占4.32%（45/1024）,42型,占4.22%（44/1024）。单一型别占总感染例数的50.53%（286/566）,多重感染以二重感染最多,占总感染例数的12.92%（158/566）。结论：陕西省商洛地区HPV感染率高,主要亚型为HPV16、HPV58、HPV66,提示HPV亚型的分析对疫苗的研发和宫颈癌的筛查及防治有指导意义。
Objective： To investigate infection status and distribution of subtypes of cervical human papillomavirus（ HPV） of 937 female of childbearing age in Shangluo,Shaanxi. Methods： All 937 cases were studied. Cervical cell specimens and 23 HPV genotypes were analyzed by PCR and reverse dot blot gene chip technology. Results： Total of556 specimens were found with positive HPV among 937 cases,the positive rate was 60. 41%（ 566 /937）. High- risk HPV infection rate was 51. 44%（ 482 /937）,low- risk HPV infection rate 26. 47%（ 248 /937）. The infection rate in 61 years old was the highest（ 88. 89%）,next was ≤20 years old（ 67. 86%）,51 ~ 60 years old（ 62. 90%）,41 ~50 years old（ 60. 29%）. However,there was no significant difference among age groups（ P〈0. 05）. The most common high- risk HPV subtype was HPV16（ 26. 58%,277 /1042,）,and next was HPV58（ 8. 93%,93 /1024）,HPV66（ 6. 53%,68 /1024）. The most common low- risk HPV subtype was HPV6（ 12. 38%,129 /1024）,next was HPV43（ 4. 32%,45 /1024） and HPV42（ 4. 22%,44 /1024）. The infection rate of single subtype was 50. 53%（ 286 /566）,the double infection was the most common in multiple infection,the infection rate was 12. 92%（ 158 /566）. Conclusion： HPV infection rate of women of childbearing age in Shangluo is higher,the more common HPV subtypes are HPV16,HPV58,HPV66,HPV genotypes detection technology provides an instruction not only for vaccine development but also prevention and treatments of cervical cancer.
出处
《现代肿瘤医学》
CAS
2016年第2期288-291,共4页
Journal of Modern Oncology
基金
陕西省科技计划发展项目资助(编号:2011K15-06-09)
关键词
人乳头瘤病毒
基因分型
宫颈癌
human papillomavirus
gene chip technique
cervical cancer
