摘要
采用木瓜蛋白酶对脱脂葡萄籽进行酶解提取蛋白质。在单因素试验基础上,考察酶用量、酶解温度、p H值、反应时间和料液比对葡萄籽蛋白质提取率的影响,采用响应面法对葡萄籽蛋白质水酶法提取条件进行了优化。结果表明,葡萄籽蛋白质的最优提取条件为:酶解温度40℃,料液比1∶25(g∶m L),酶用量4%,酶解时间45 min。在此优化条件下,葡萄籽蛋白质提取率为67.85%。
Protein was extracted from defatted grape seeds by hydrolysis of papain. Base on single factor experiments, the effect of enzyme addition, enzymolysis temperature, pH, time and solid-liquid ratio on grape seed protein extraction rate was investigated. Aqueous enzymatic method extrac- tion conditions of grape seeds protein were optimized by response surface methodology. Results indicated that the optimum extraction conditions were as follows： enzymolysis temperature 40 ℃, solid-liquid ratio 1：25 （g：ml）, enzyme addition 4% and enzymolysis time 45 min. Under this condition, the grape seed protein extraction rate was 67.85%.
出处
《中国酿造》
CAS
北大核心
2015年第12期122-126,共5页
China Brewing
基金
吉林省大学生创新创业训练计划项目(吉教高字[2015]17号)
关键词
水酶法
葡萄籽蛋白质
响应面法
提取条件
aqueous enzymatic method
grape seeds protein
response surface methodology
extraction conditions
