摘要
为了解不同类型异质雄性不育小麦的易恢性差异和不同恢复系的恢复力差异,采用特异性分子标记及醇溶蛋白的A-PAGE检测方法对5种类型同核异质不育系(K706A、Va706A、Ju706A、C6706A、U706A,分别为K、Va、Ju、C6和U型)、保持系(706B)及恢复系(6521-2、LK783、1321、223原-9、9023晚、X197、宿7078、宿968、中国春、WM5-5)进行1B/1R类型鉴定,同时对不同杂交组合F1(不育系/恢复系)的自交结实率进行异地(杨凌和三原)分析。结果表明,Va706A、Ju706A、C6706A、U706A、706B为1BL/1RS易位纯合体,宿968、宿7078、LK783、1321、6521-2、WM5-5、X197、223原-9为非1BL/1RS易位材料,K706A、9023晚为1BL/1RS易位杂合体。5种细胞质雄性不育系中,K型的易恢性最好,Ju型和U型居中,C6型恢复能力最差。10个恢复系中,LK783和中国春在三原和杨凌恢复度均较高,宿968的恢复能力最弱,但LK783的变异幅度较大,中国春的变异幅度较小,说明中国春恢复能力好且稳定。此外,不育系与恢复系间存在明显的互作关系。
In order to provide reliable basis for the selection of male sterile lines,restorer lines and hybrid combinations in wheat,five isonucleus male sterile lines（K706A,Va706 A,Ju706A,C6706 A,U706A）,maintainer line（706B）and restorer line（6521-2,LK783,1321,223Yuan-9,9023 Wan,X197,Su 7078 and Su 968,Chinese Spring,WM5-5）were tested as materials,using specific markers and the A-PAGE method for detecting the Gliadin to distinguish 1BL/1RS type and non 1BL/1RS type of wheat.The results showed that Va706 A,Ju706A,C6706 A,U706Aand 706 B were homozygous 1BL/1RS;6521-2,LK783,1321,223Yuan-9,X197,Su 7078 and Su 968,Chinese Spring,WM5-5were non-1BL/1RS;K706A,9023 Wan were heterozygous 1BL/1RS.50 hybrids,derived from the crosses between five sterile lines and ten restorer lines,were conducted to study its restoring rates and analyze its correlation and path coefficient of yield.The results showed that among the five sterile lines,K has the easiest restoration of fertility,followed by Ju and U,and C6 is the last one.Among the ten restorer lines,LK783 and Chinese Spring are a group of high restorer lines.However,the amplitude of LK783 is larger,and that of Chinese Spring is small,which indicates that the recovery ability of China Spring is good and stable.Su 968 has lower restoring ability.There are interactive effects between sterile linesand restorer lines.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2015年第12期1676-1684,共9页
Journal of Triticeae Crops
基金
国家自然科学基金项目(31271792)
陕西省农业攻关项目(2014K02-04-01)
西北农林科技大学唐仲英育种基金项目
关键词
小麦
细胞质雄性不育
生物学特性
育性恢复
Wheat
Cytoplasmic male sterility
Biological characteristics
Fertility restoration
