摘要
目的探讨兔肝缺血/再灌注损伤的机制及N-乙酰半胱氨酸对兔肝的保护作用。方法选40只实验兔,将其随机分为肝缺血/再灌注组(I/R组)和肝缺血/再灌注+N-乙酰半胱氨酸组(I/R+NAC组),于门静脉阻断前(T_0)、门静脉开放即刻(T_1)、开放后2 h(T_2)、开放后5 h(T_3)、开放后7 h(T_4)时间点记录心率(HR)、平均动脉压(MAP),分别测定各组血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、丙二醛(MDA)、超氧化物岐化酶(SOD),并检测肝细胞凋亡数。结果与T_0比较,两组T_1、T_2、T_3、T_4时间点ALT、AST及MDA显著升高,SOD明显降低;与I/R组比较,I/R+NAC组T_1、T_2、T_3、T_4时间点ALT、AST及MDA显著下降,SOD明显升高,且I/R+NAC组T_3、T_4时凋亡细胞数减少。结论 NAC对兔肝缺血/再灌注损伤有保护作用,可能与NAC清除氧自由基,抑制肝细胞凋亡有关。
Objective To explore the protective effect of N-acetylcysteine on rabbit hepatic ischemia/reperfusion injury.Methods 40 experimental rabbits were selected and randomly divided into hepatic ischemia reperfusion group（I/R group）and hepatic ischemia reperfusion plus N-acetylcysteine group（I/R＋NAC group）.Heart rate（HR）and mean arterial pressure（MAP）were recorded before portal vein occlusion（T_0）,when portal vein released（T_1）,two hours after portal vein released（T_2）,five hours after portal vein released（T_3）and seven hours after portal vein released（T4）.Alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,malonaldehyde（MDA）,superoxide dismutase（SOD）and apoptosis cells were tested.Results Compared with T_0,ALT,AST and MDA at T_1,T_2,T_3 and T_4increased significantly and SOD decreased significantly in both groups.Compared with I/R group,ALT,AST and MDA at T1,T2,T3 and T4in I/R＋NAC group decreased significantly and SOD increased significantly;apoptosis cells decreased at T_3 and T_4 in I/R ＋NAC group.Conclusion NAC can protect rabbits from hepatic ischemia/reperfusion injury probably due to oxygen-free radical elimination and hepatocyte apoptosis suppression.
出处
《中国当代医药》
2015年第36期7-10,共4页
China Modern Medicine
