摘要
目的探讨转化生长因子-β1（transforming growth factor β1，TGF-β1）和转化生长因子-β2（transforming growth factor β2，TGF-β2）在分泌性中耳炎（secretory otitis media，SOM）发病过程中的作用。方法采用双抗体夹心酶联免疫吸附试验法（enzyme linked immunosorbent assay ELISA）检测56例（61只耳）SOM患者中耳腔积液、外周血清及32例正常人外周血清中TGF-β1、TGF-β2的表达水平。结果在SOM患者中耳积液、血清及正常人血清中TGF-β1、TGF-β2的阳性检出率均为100％；急性SOM患者中耳积液中TGF-β1浓度低于慢性患者（P〈0．05），而TGF-β2浓度高于慢性患者（P〈0．05）；急性SOM患者血清中TGF-β1含量与正常人比无显著性差异（P〉0．05），TGF-β2含量与正常人比有显著性差异（P〈0．05）；慢性SOM患者血清中TGF-β1含量与正常人比有显著性差异（P〈0．05），而TGF-β2含量与正常人比无显著性差异（P〉0．05）。结论TGF-β1、TGF-β2均参与SOM的发生发展过程，TGF-β1在SOM的慢性病程中发挥作用，TGF-β2主要在SOM的早期发挥作用。
Objective To investigate the role of transforming growth factor β1 （ TGF - β1 ） and transforming growth factor β2（ TGF - β2）in the pathogenesis of secretory otitis media（ SOM ）. Methods The expression levels of TGF - β1 and TGF - β2 in middle ear effusions（MEEs） and blood plasma were measured in 56 patients（61 ears） with SOM as well as 32 normal volunteers using enzyme linked immunosorbent assay（ELISA）. Results The positive rates of TGF - β1 and TGF - β2 in MEEs and plasma in SOM patients as well as normal plasma were all 100%. The con- centration of TGF - β1 in MEEs in patients with acute SOM was significantly lower than that in patients with chronic SOM（P 〈 0.05 ）. The concentration of TGF - β2 in MEEs in patients with acute SOM was significantly higher than that in patients with chronic SOM（P 〈0.05）. There was no significant difference in the concentration of TGF- β1 in plasma between patients with acute SOM and normal volunteers （ P 〉 0.05 ）. There was significant difference in the concentration of TGF -β2 in plasma between patients with acute SOM and normal volunteers （ P 〈0. 05 ）. There was significant difference in the concentration of TGF -β1 in plasma between patients with chronic SOM and normal volunteers（ P 〈0.05）. There was no significant difference in the concentration of TGF -β2 in plasma between patients with acute SOM and normal volunteers （P 〉 0.05）. Conclusion TGF - β1 and TGF -β2 both participate in pathogenesis of SOM. TGF - β1 may play a role in the chronic course of SOM and TGF - β2 may be an important role in the early stage of SOM.
出处
《医学新知》
CAS
2015年第6期381-382,385,共3页
New Medicine
基金
武汉市卫生局科研资助项目
